生物膜形成对植物乳杆菌PG3-1耐受性及功能基因表达的影响  

The effection of Lactobacillus plantarum PG3-1 biofilm on the tolerance and gene expression

作　　者：张国丽[1] 杨陈文 余茜[1] 陈安均[1] 刘书亮[1,2] 敖晓琳[1,2] ZHANG Guo-li;YANG Chen-wen;YU Xi;CHEN An-jun;LIU Shu-liang;AO Xiao-lin(College of Food Science , Siehuan Agricultural University, Ya＇an 62500, China;Research Institute of Food Processing and Security ,Siehuan Agricultural University, Ya＇an 62500)

机构地区：[1]四川农业大学食品学院,四川雅安625000 [2]四川农业大学食品加工与安全研究所,四川雅安625000

出　　处：《食品与发酵工业》2018年第4期29-34,共6页Food and Fermentation Industries

基　　金：四川省科技支撑计划项目(2015FZ0001);四川省科技支撑计划(2012N0002);国家自然科学基金(31171726)

摘　　要：研究了植物乳杆菌PG3-1生物膜形成后对其耐受性以及功能基因表达的影响。采用平板计数及实时荧光定量PCR对其耐受性以及功能基因相对表达量进行测定。结果表明,植物乳杆菌生物膜形成后对温度、酸碱、盐度耐受性均有不同程度的提高。植物乳杆菌生物膜形成过程中发生热应激、酸应激、盐应激等应激行为,Gro EL、Dan K热应激基因的相对表达量达到3.22和1.53。This artcle had studied the effection of Lactobacillus plantarum PG3-1 biofilm formation on the expression of stress gene and tolerance. Quantitative real-time PCR and plate count are used for the tolerance and gene expression. The results show that mature Lactobacillus plantarum PG3-1 biofilm had stronger tolerance to temperature,acid-base and salinity. The Stress behavior such as heat stress,salt stress occured in the process of Lactobacillus plantarum PG3-1 biofilm formation,and the relative expression of Gro EL,Dan K gene was 3. 22 and 1. 53.

关 键 词：植物乳杆菌 生物膜 耐受性 实时荧光定量PCR 

分 类 号：Q933[生物学—微生物学] TS201.3[轻工技术与工程—食品科学]