黄秋葵花提取物不同极性部位抗氧化活性的研究  

Study on the Antioxidant Activity of Different Polarity Fractions of Okra Flowers Extract

作　　者：郭溆[1] 李文刚 田硕 曹宏杰 王维婷[1] 刘超[1] 程安玮[1] 王新坤[1] 孙金月[1] GUO Xu;LI Wen-gang;TIAN Shuo;CAO Hong-jie;WANG Wei-ting;LIU Chao;CHEN An-wei;WANG Xin-kun;SUN Jin-yue(Institute of Agro-Food Science and Technology, Shandong Academy of Agricultural Sciences/ KeyLaboratory of Agro-Products Processing Technology of Shandong Province/Key Laboratory of Novel FoodResources Processing, Ministry of Agriculture, Jinan 250100, Shandong, China;Experimental BaseService Center, Shandong Academy of Agricultural Sciences, Jinan 250100, Shandong, China;JinanAnimal Product Quality and Safety Monitoring Center, Jinan 250000, Shandong, China)

机构地区：[1]山东省农业科学院农产品研究所/山东省农产品精深加工技术重点实验室/农业部新食品资源加工重点实验室,山东济南250100 [2]山东省农业科学院试验基地服务中心,山东济南250100 [3]济南市畜产品质量安全监测中心,山东济南250000

出　　处：《食品研究与开发》2018年第10期17-21,共5页Food Research and Development

基　　金：山东省农业科学院青年科研基金(2015YQN56)

摘　　要：为确定黄秋葵花提取物的抗氧化活性,采用有机溶剂萃取法将黄秋葵花提取物分为乙酸乙酯相和水相2个不同极性部位,测定不同极性部位提取物中总黄酮及多酚的含量,分析其还原能力、(1,1-diphenyl-2-picrylhydrazyl)DPPH自由基清除能力、超氧阴离子自由基清除能力和羟自由基清除能力,比较黄秋葵花不同极性部位提取物的抗氧化作用。结果表明,黄秋葵花提取物的不同极性部位均有抗氧化活性作用,其中乙酸乙酯相部位提取物的羟自由基清除能力和超氧阴离子清除能力均强于水相提取部位,而水相提取部位的还原力和DPPH自由基清除能力强于乙酸乙酯相部位提取物。本研究将为黄秋葵花抗氧化功能食品的研究与开发提供理论依据。The objective of this study was to determine antioxidant activity of okra flowers extract. The extracts were prepared by ethanol solvent extraction and divided into different polarity fractions（ethyl acetate and water）. The contents of total flavonoid and phenolic in different polarities were determined respectively. Their antioxidant activities were estimated by the reducing power and the strong scavenging abilities to 1-dipheny1-2-picrylhydrazy1,hydroxyl free radical and superoxide anion. The results showed that both polarity fractions had the antioxidant function. In the ethyl acetate fraction,the superoxide anion and hydroxyl free radical scavenging ability were stronger than the water fraction. However,reducing power and 1-dipheny1-2-picrylhydrazy1 scavenging capacity is weaker than the water fraction. This study provides a theoretical basis for further research and development of functional foods of okra flowers.

关 键 词：黄秋葵花 极性部位 总黄酮 多酚 抗氧化活性 

分 类 号：TS201.2[轻工技术与工程—食品科学]