大豆11S球蛋白热力学特性和溶液性质与表面疏水性关系研究  

Study on relationship between the thermodynamic characters/solution qroperties and surface hydrophobicity of soybean β-Conglycinin(11S)

作　　者：李丹[1] 魏冬旭 贾烨[3] 刘春雷[1] 江连洲[4] LI Dan ,WEI Dong-xu2 ,JIA Ye3 ,LIU Chun-lei ,JIANG Lian-zhou4(1.Ningde Normal University, Ningde 352100, China ; 2.Heilongjiang Entry-Exit Inspection and Quarantine Bureau, Harbin 150001 , China 3. Hangzhou Wahaha Group Co., Ltd., Hangzhou 310018, China ; 4.College of Food Science, Northeast Agricuhural University, Harbin 150030, Chin)

机构地区：[1]宁德师范学院,福建宁德352100 [2]黑龙江出入境检验检疫局,黑龙江哈尔滨150001 [3]杭州娃哈哈集团有限公司,浙江杭州310018 [4]东北农业大学食品学院,黑龙江哈尔滨150030

出　　处：《食品工业科技》2018年第10期46-50,57,共6页Science and Technology of Food Industry

基　　金：国家自然科学基金项目(C200101);福建省科技厅重点项目(2014N0007);宁德师范学院校级课题(2016Z07;2016Z08)

摘　　要：以6个具有代表性的大豆品种制备的11S球蛋白为实验对象,研究大豆11S球蛋白热力学特性、溶解性和溶液性质与表面疏水性的关系。采用ANS荧光探针法测定表面疏水性,差示扫描量热法分析热力学特性,体积排阻-凝胶色谱法分析溶液的分子量及其分布,应用动态光散射技术分析流体动力学半径及其分布,Zeta-电位仪测定蛋白ξ-电位。结果表明,大豆11S球蛋白的表面疏水性与变性温度(p=0.010)和变性焓(p=0.012)均呈现显著负相关;大豆11S球蛋白的溶解性与表面疏水性极显著负相关(p=0.003);大豆11S球蛋白溶液可溶性聚集物的平均分子量与表面疏水性极显著正相关(p=0.005),平均直径与表面疏水性极显著正相关(p=0.007),ξ-电位绝对值与表面疏水性显著负相关(p=0.034)。Taking β-Conglycinin（ 11 S） which was prepared with six representative soybean varieties as the object,Study the relationship between the thermodynamic characters/solution properties and surface hydrophobicity. Surface hydrophobicity of11 S was determined by ANS fluorescent probe method,thermodynamic characteristics was analyzed by differential scanning calorimetry,molecular weight distribution was determined by size exclusion gel chromatography,hydrodynamic radius was analyzed by dynamic light scattering analyzer and ξ-potential was determined by Zeta-potential meter.The results showed that there was significantly negative correlation between surface hydrophobicity of 11 S and thermal denaturation temperature（ p =0.010）/denaturation enthalpy（ p = 0.012）,and negatively correlated with solution properties（ p = 0.003）. There was positive correlation between surface hydrophobicity of 11 S and average molecular weight（ p = 0.005）/average diameter（ p = 0.007）,and negative correlation with ξ-potential absolute value（ p = 0.034）.

关 键 词：大豆11S球蛋白 热力学特性 分子量分布 流体动力学半径 ξ-电位 表面疏水性 

分 类 号：TS201.7[轻工技术与工程—食品科学]