L-苹果酸降解菌酿酒酵母降酸功能影响因素分析  

Analysis the Influence Factors and Optimization the Deacidification Conditions of the L-malic Acid Degradation Capability of Saccharomyces cerevisiae

作　　者：王英[1] 周剑忠[1] 夏秀东 胡彦新[1] 李莹[1] 黄自苏[1] WANG Ying;ZHOU Jianzhong;XIA Xiudong;HU Yanxin;LI Ying;HUANG Zisu(Institute Ago-Product Processing,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,China)

机构地区：[1]江苏省农业科学研究院农产品加工研究所,江苏南京210014

出　　处：《食品与生物技术学报》2018年第10期1067-1072,共6页Journal of Food Science and Biotechnology

基　　金：江苏省科技支撑计划项目(BE2014302);江苏省自主创新项目(CS(15)1026)

摘　　要：为了使降酸酿酒酵母FM-S-115在果酒发酵过程中发挥优良的降解L-苹果酸的功能,利用单因素实验分析影响FM-S-115菌株降酸功能的主要因素,利用中心组合设计建立FM-S-115菌株降酸率与主要影响因素之间的数学模型,获取最佳降酸条件。研究结果显示,发酵温度、接种量和SO_2质量浓度为主要影响因素,最优条件为发酵温度26.26℃,接种量(体积分数)为5.15%,SO_2质量浓度为81.08 mg/L。采用上述优化后的条件,FM-S-115的降酸率达38.11%,与37.98%接近,说明该实验模型的可操作性高。The Saccharomyces cerevisiae FM-S-115 strain has good function in degradation L-malic acid,in order to play a good role in degradation L-malic acid of FM-S-115 strain during wine fermentation, the main influence factors and mathematical relational model between deacidification ratio and main influence factors were analyzed using univariate analysis and Central Composite Design experiment. The results showed that fermentation temperature,inoculation amount and SO2 concentration were the main influence factors on the deacidification ratio of FM-S-115 strain,the optimum conditions were the fermentation temperature 26.26℃ ,the inoculation amount 5.15% and the SO2 concentraion 81.08 mg/L. The experimental values of deacidification ratio of FM-S-115 strain was 38.11%, which well matched with the response surface methodology predicted values.

关 键 词：L-苹果酸 酿酒酵母 降酸功能 响应面设计 回归模型 

分 类 号：TS201.1[轻工技术与工程—食品科学]