米根霉薯渣固态发酵产L(+)-乳酸的研究  

L(+)-Lactic Acid Production by Rhizopus Oryzae PW352 in the Solid-state Fermentation of Sweet Potato Pulp

作　　者：沈寿国[1] 刘献文[1,2] 张洁[1] 潘仁瑞[1] 葛春梅[1] 蔡敬民[1,2] SHEN Shou-guo;LIU Xian-wen;ZHANG Jie;PAN Ren-rui;GE Chun-mei;CAI Jing-min(Department of Biological and Environmental Engineering,Hefei University,Hefei 230601;School of Life Sciences,Anhui Agricultural University,Hefei 230036,China)

机构地区：[1]合肥学院生物与环境工程系,合肥230601 [2]安徽农业大学生命科学学院,合肥230036

出　　处：《合肥学院学报（综合版）》2018年第5期60-64,96,共6页Journal of Hefei University：Comprehensive ED

基　　金：安徽省优秀青年科技基金项目(04043051);合肥学院研究发展基金(06KY001ZR)资助

摘　　要：以甘薯渣为原料,对米根霉PW352固态发酵产L(+)-乳酸的发酵条件进行了研究。采用单因素试验、均匀设计、正交设计等方法确定最优固态发酵培养基组成为:干薯渣10g/250ml三角瓶、(NH4)2SO41. 5%、MgSO40. 2%、Zn SO40. 05%、MnCl20. 05%、葡萄糖0. 5%、吐温80 0. 05%,在接种量为1. 0×107个孢子/10g干培养基、湿度70%、中和剂碳酸钙30%、36℃条件下,发酵72h产L(+)-乳酸达38. 36(±0. 51)%(w/w),对淀粉糖的转化率为62. 5%。Production of L（ ＋ ） -lactic acid by Rhizopus oryzae PW352 in the solid-state fermentation of sweet potato pulp was investigated by means of orthogondl design, uniform design. A growth condition with 10g sweet potato pulp/250ml flask, （ NH4） 2SO4 1.5% ,MgSO4 0.2% ,ZnSO40.05% ,MnCl2 0. 05% ,glucose 0.5% ,Tween 80 0.05% ,CaCO3 30% , at moisture content of 65% - 70% ,volume of seeds 1.0 × 10^7and 36℃ was favourable for lactic acid fermentation, resulting in lactic acid production of 38.36（ ±0.51 ） % （w/w） produced in 72 h fermentation.

关 键 词：米根霉 L-乳酸 甘薯渣 固态发酵 

分 类 号：Q557[生物学—生物化学]