琼胶寡糖体外清除自由基活性的研究  被引量：34

Antioxidant abilities of agar oligosaccharides</em> <p class='article-op'> <span id="bdshare" class="bdshare_t bds_tools get-codes-bdshare baidufx"><span><span class="bds_more" style="display: block; width: 62px; height: 29px; background: url(/images/share.gif) no-repeat!important; padding: 0;"></span></span></span> </p> <p class="article-source"> <a href ='/article/read.aspx?id=6820473' class='btnTitle btnIsView' target='_blank'><img alt='在线阅读' src='/images/btn-read.png'/></a><a class='btnTitle btnIsView' href="javascript:showdown('vrMAfK/CrCQc5cbU7xZJcmVHIzdqzNta7ZoYphgeJjA=')"><img alt="下载全文" src="/images/btn-down.png" /></a> <input id="hiddowninfo" type="hidden" value="vrMAfK/CrCQc5cbU7xZJcmVHIzdqzNta7ZoYphgeJjA="/> </p> <div class="collect" style="display: none"> <h5>我的收藏</h5> <div class="cnt"> <p> 名 称：<input type="text" name="collTitle" id="collSumTitle" /> </p> <p> 注 释：<input type="text" name="collRemark" id="collSumRemark" /> </p> <p style="text-align: center;"> <a href="javascript:void(0)" onclick="g_collectSumOk();"> <img width="65" height="17" src="/images_new/collect_ok.png" alt="完成" /></a> <a href="javascript:void(0)" onclick="g_collectSumCancel();"> <img width="65" height="17" src="/images_new/collect_no.png" alt="取消" /></a> </p> </div> </div> </div> <form name="wxTransfer" method="post"> <input type="hidden" name="lid" value="" /> <input type="hidden" name="sip" value="" /> <input type="hidden" name="date" value="" /> <input type="hidden" name="co" value="" /> <input type="hidden" name="Title_C" value="" /> <input type="hidden" name="Writers" value="" /> <input type="hidden" name="Name_C" value="" /> <input type="hidden" name="Num" value="" /> <input type="hidden" name="Years" value="" /> <input type="hidden" name="Volumn" value="" /> <input type="hidden" name="GCH" value="" /> <input type="hidden" name="Vol" value="" /> <input type="hidden" name="BookID" value="" /> <input type="hidden" name="SubjectNum" value="" /> <input type="hidden" name="SpecialNum" value="" /> <input type="hidden" name="mirrorName" value="" /> <input type="hidden" name="UserID" value="" /> <input type="hidden" name="UserName" value="" /> <input type="hidden" name="sourceID" value="" /> </form> <script type="text/javascript" src="/scripts/transfer.min.js"></script> <div class="article-detail"> <p class='author'><strong>作　　者：</strong><a href='/writer/article.aspx?id=100000009678890' target='_blank' ><b class='type_ico_id'></b></a><a href='/zk/search.aspx?key=A%3d%5b%e8%b5%b5%e9%9b%aa%5d' title='赵雪[1]'>赵雪[1]</a> <a href='/writer/article.aspx?id=100000007822797' target='_blank' ><b class='type_ico_id'></b></a><a href='/zk/search.aspx?key=A%3d%5b%e8%96%9b%e9%95%bf%e6%b9%96%5d' title='薛长湖[1]'>薛长湖[1]</a> <a href='/writer/article.aspx?id=100000001165793' target='_blank' ><b class='type_ico_id'></b></a><a href='/zk/search.aspx?key=A%3d%5b%e5%be%90%e5%bc%ba%5d' title='徐强[1]'>徐强[1]</a> <a href='/writer/article.aspx?id=100000012292716' target='_blank' ><b class='type_ico_id'></b></a><a href='/zk/search.aspx?key=A%3d%5b%e8%ae%b8%e5%8a%a0%e8%b6%85%5d' title='许加超[1]'>许加超[1]</a> <a href='/writer/article.aspx?id=100000005711051' target='_blank' ><b class='type_ico_id'></b></a><a href='/zk/search.aspx?key=A%3d%5b%e6%9d%8e%e5%85%86%e6%9d%b0%5d' title='李兆杰[1]'>李兆杰[1]</a> <a href='/writer/article.aspx?id=100000012061548' target='_blank' ><b class='type_ico_id'></b></a><a href='/zk/search.aspx?key=A%3d%5b%e6%9e%97%e6%b4%aa%5d' title='林洪[1]'>林洪[1]</a> <em> </em></p><p class='organ'><strong>机构地区：</strong><a href='/organ/article.aspx?id=14999&subid=&showname=&searchtype=' target='_blank'><b class='type_ico_id'></b></a><a href='/zk/search.aspx?key=S%3d%e9%9d%92%e5%b2%9b%e6%b5%b7%e6%b4%8b%e5%a4%a7%e5%ad%a6%e6%b0%b4%e4%ba%a7%e5%ad%a6%e9%99%a2' title='[1]青岛海洋大学水产学院,山东青岛266003'>[1]青岛海洋大学水产学院,山东青岛266003</a> <em> </em></p><p class='from'><strong>出　　处：</strong><a href='/journal/summary.aspx?gch=98270X' target='_blank'><b class='type_ico_id'></b></a><a href='/zk/search.aspx?key=J%3d%5b%e4%b8%ad%e5%9b%bd%e6%b0%b4%e4%ba%a7%e7%a7%91%e5%ad%a6%5d' title='中国水产科学'>《中国水产科学》</a>2002年第3期280-282,共3页<em>Journal of Fishery Sciences of China</em></p><p class='fund'><strong>基　　金：</strong>山东省优秀中青年科学家奖励基金项目</p><p class='abstrack'><strong>摘　　要：</strong>在不同条件下对琼胶进行酸水解,得琼胶寡糖A1(以二、四糖为主)和A2(以己糖、辛糖为主)采用化学发光法和DPPH体系分别研究A1、A2对O2·-、·OH和DPPH3种自由基的清除作用。结果表明,A1、A2对3种自由基都有较好的清除作用,而且清除活性随糖浓度的增加而加强。在O2·-体系中,A2的IC50为0.54mg/mL,效果要好于A1(IC50为0.8mg/mL)。在·OH体系中,A2对·OH的清除作用比A1效果好,IC50分别为1.2和2.3mg/mL。2个体系比较发现A1、A2对O2·-的清除作用高于对·OH的清除作用。在DPPH体系中,A1、A2对DPPH有一定的清除,但A1较A2效果好,IC50分别为4和6 4mg/mL。由此可见,琼胶寡糖在不同体系中清除自由基的活性不同。琼胶寡糖在抗氧化方面具有很好的应用价值。<em><Abstrcat> The agar oligosaccharides A1(mainly with disaccharidae and tetrasaccharide) and A2 (mainly with hexose and octoses) were prepared from agar under different acid hydrolysis conditions. Using the methods of Luminesecence and DPPH system, the scavenging effects of A1 and A2 on O2·-,·OH and DPPH are determined that A1 and A2 have strong scavenging effects on O2·- with the IC50 at 0.8 and 0.54 mg/mL. The scavenging activities of A1 and A2 on ·OH are lower than those on O2·- and their IC50 on ·OH are 2.3 and 1.2 mg/mL. A2 has better scavenging ability on O2·-and ·OH than A1. A1 and A2 have certain scavenging activities on DPPH and A1 shows higher scavenging ability than A2, which is quite different from the results in O2·- and · OH. But their abilities are much lower than probucol and Vc, which have very active hydrogens and good scavenging activity on DPPH. It suggests that agar oligosaccharides A1 and A2 have different scavenging abilities in different free radical assays.</em></p><p class='subject'><strong>关 键 词：</strong><a href='/subject/article.aspx?id=3167284' target='_blank'><b class='type_ico_id'></b></a><a href='/zk/search.aspx?key=K%3d%5b%e7%90%bc%e8%83%b6%e5%af%a1%e7%b3%96%5d' title='琼胶寡糖'>琼胶寡糖</a> <a href='/subject/article.aspx?id=9007048' target='_blank'><b class='type_ico_id'></b></a><a href='/zk/search.aspx?key=K%3d%5b%e8%b6%85%e6%b0%a7%e9%98%b4%e7%a6%bb%e5%ad%90%e8%87%aa%e7%94%b1%e5%9f%ba%5d' title='超氧阴离子自由基'>超氧阴离子自由基</a> <a href='/subject/article.aspx?id=3738281' target='_blank'><b class='type_ico_id'></b></a><a href='/zk/search.aspx?key=K%3d%5b%e7%be%9f%e5%9f%ba%e8%87%aa%e7%94%b1%e5%9f%ba%5d' title='羟基自由基'>羟基自由基</a> <a href='/subject/article.aspx?id=8369282' target='_blank'><b class='type_ico_id'></b></a><a href='/zk/search.aspx?key=K%3d%5bDPPH%e8%87%aa%e7%94%b1%e5%9f%ba%5d' title='DPPH自由基'>DPPH自由基</a> <a href='/zk/search.aspx?key=K%3d%5b%e6%b8%85%e9%99%a4%e4%bd%9c%e7%94%a8%5d' title='清除作用'>清除作用</a> <a href='/subject/article.aspx?id=3147523' target='_blank'><b class='type_ico_id'></b></a><a href='/zk/search.aspx?key=K%3d%5b%e6%b8%85%e9%99%a4%e6%b4%bb%e6%80%a7%5d' title='清除活性'>清除活性</a> </p><p class='class'><strong>分 类 号：</strong><a href='/zk/search.aspx?key=C%3dQ533' title='Q533'>Q533</a>[<a href = '/domain/article.aspx?id=105' >生物学—生物化学</a>] </p> </div> <div id="content_wzxg" name="wzxg" class="article-graph"> <object width="695" height="250" classid="clsid:D27CDB6E-AE6D-11cf-96B8-444553540000" id="FlashID"> <param value="../flash/relation.swf" name="movie"> <param value="high" name="quality"> <param value="opaque" name="wmode"> <param value="6.0.65.0" name="swfversion"> <param value="dataUrl=/ajax/articlerelativegraph.ashx?id=6820473" name="flashVars"> <embed width="695" height="250" src="../flash/relation.swf" type="application/x-shockwave-flash" quality="high" wmode="opaque" 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