酒酒球菌β-葡萄糖苷酶活性与耐酸胁迫能力的相关性分析  

Correlation between β-Glycosidase Activity and Acid Stress Tolerance in Oenococcus oeni

作　　者：陈其玲[1] 任晓宁[1] 王玲[1] 田雨[1] 赵美静[1] 宋巧智 刘树文[1,2,3] CHEN Qiling;REN Xiaoning;WANG Ling;TIAN Yu;ZHAO Meijing;SONG Qiaozhi;LIU Shuwen(College of Enology, Northwest A&F University, Yangling 712100, China;Shaanxi Engineering Research Center for Viti-Viniculture, Yangling 712100, China;Heyang Experimental Demonstration Station, Northwest A&F University, Yangling 715300, China)

机构地区：[1]西北农林科技大学葡萄酒学院,陕西杨凌712100 [2]陕西省葡萄与葡萄酒工程技术中心,陕西杨凌712100 [3]西北农林科技大学合阳葡萄试验示范站,陕西渭南715300

出　　处：《食品科学》2017年第2期115-120,共6页Food Science

摘　　要：目的:β-葡萄糖苷酶是葡萄酒中结合态香气物质释放的关键酶,但其活性受诸多因素的影响。本研究旨在从酒酒球菌自身耐酸能力的视角去分析评估菌株糖苷酶活性,探索酸胁迫下不同耐酸表型酒酒球菌与其β-葡萄糖苷酶活性之间存在的相关性关系。方法:结合利用离子注入诱变与胁迫环境筛选的方式,获得耐酸(p H 3.0)、酸敏(p H 9.0)突变酒酒球菌菌株,对其β-葡萄糖苷酶的活力进行测定,并筛选3组酸胁迫下表型差异大的β-葡萄糖苷酶基因送样测序。结果:耐酸突变酒酒球菌的β-葡萄糖苷酶酶活力是出发菌株的2~4倍,是相应酸敏突变株的2~7倍。测序结果显示,除菌株a3的β-葡萄糖苷酶基因在108位(G置换成C)和1 232(A置换成T)位处发生了突变外,其余菌株β-葡萄糖苷酶的基因均未发生改变。结论:酒酒球菌β-葡萄糖苷酶活性与菌株酸胁迫能力显著相关(P<0.05)。耐酸胁迫能力越强的菌株,β-葡萄糖苷酶活性越高。Objective: β-Glycosidase plays a crucial role in the hydrolysis of aglycones and subsequent release of grapederived aroma compounds in wine. However, β-glycosidase activity is influenced by many factors. This study was designed taking into account the acid tolerance of Oenococcus oeni to assess and correlate their glucosidase activities with acid stress tolerance. Methods: The cells of O. oeni were implanted with N+ ion to obtain acid-tolerant (pH 3.0) and acid-sensitive (pH9.0) mutants. Besides, β-glycosidase activities of all the tested strains were determined. Three groups of variant O. oeniwere selected and their β-glycosidase genes were sequenced. Results: The acid-tolerant mutants harbored a significantlyincreased β-glucosidase activity that was 2–4 times higher than that of the original strains, and 2–7 times higher than that ofthe corresponding acid-sensitive mutants. Sequencing results of eight representative amplicons indicated that all the mutantsand their parental strains were identical to each other, except a3 mutant, which formed two base transversions at 108 gene locus (G→C) and at 1 232 locus (A→T). Conclusion: Correlation analysis indicated that O. oeni β-glycosidase activity was significantly positively related to acid stress resistance (P < 0.05).

关 键 词：酒酒球菌 离子注入诱变 酸胁迫 Β-葡萄糖苷酶 相关性分析 

分 类 号：Q815[生物学—生物工程]