高压脉冲电场对抗氧化肽荧光特性的影响  

Effect of Pulsed Electric Field (PEF) on Fluorescence Characteristics of Antioxidant Peptides

作　　者：王莹[1] 刘静波[2] 邢杰[2] 李幸芳[2] 殷涌光[1] WANG Ying;LIU Jingbo;XING Jie;LI Xingfang;Yin Yongguang(College of Biological and Agricultural Engineering, Jilin University, Changchun 130025, China;College of Food Science and Engineering, Jilin University, Changchun 130062, China)

机构地区：[1]吉林大学生物与农业工程学院,吉林长春130025 [2]吉林大学食品科学与工程学院,吉林长春130062

出　　处：《食品科学》2017年第3期53-58,共6页Food Science

基　　金：国家高技术研究发展计划(863计划)项目(2013AA102206-5)

摘　　要：以前期研究所得的Gln-Trp-Phe-Met（QWFM，652.78）和Lys-Trp-Phe-Met（KWFM，610.78）抗氧化四肽为研究对象，探究高压脉冲电场技术（pulsedelectricfield，PEF）对抗氧化肽荧光特性的改变。研究表明，在PEF作用下QWFM和KWFM的荧光强度发生了不同程度的改变，由于两条结构相似的抗氧化四肽中色氨酸前端所连接的氨基酸不同，在相同的PEF处理条件下，QWFM的荧光强度变化更为显著，在电场频率为1800Hz和电场强度为15kV/cm时，其荧光强度变化最显著。通过监测经PEF处理后2h抗氧化四肽荧光强度的变化，发现抗氧化四肽荧光强度的变化随着时间的延长而逐渐减弱。通过圆二色谱分析和核磁共振波谱技术分析发现维持QWFM中β-折叠的氢键含量有所改变从而导致了β-折叠结构的含量有所减少。这些变化表明PEF技术可能通过改变抗氧化肽的化学结构而改变其荧光特性，为PEF技术应用于抗氧化肽的研究提供了理论基础。Two antioxidant peptides obtained in our previous work, Gln-Trp-Phe-Met (QWFM, 652.78) and Lys-Trp-Phe-Met (KWFM, 610.78), were used to explore the effect of pulsed electric field (PEF) on their fluorescence intensity. The results showed that the fluorescence intensity of both antioxidant peptides, which differed by N-terminal amino acid residues,especially QWFM, was changed after PEF treatment. The most significant change in fluorescence intensity of QWFM was observed at electric pulse frequency of 1 800 Hz and field intensity of 15 kV/cm. The fluorescence intensity of antioxidant peptides decreased as time elapsed after PEF treatment. The circular dichroism (CD) and nuclear magnetic resonance (NMR) spectra showed changed hydrogen bonds maintaining the β-sheet in QWFM and thus reduced β-sheet content. These results suggested that the chemical structure of antioxidant peptides and consequently their properties could be changed by PEF,which will provide a theoretical foundation for further exploitation and utilization of antioxidant peptides.

关 键 词：高压脉冲电场 抗氧化肽 荧光强度 化学结构 

分 类 号：TS201.4[轻工技术与工程—食品科学]