红枣色素对H_2O_2诱导血管内皮细胞损伤的保护作用  

Protective Effect of Chinese Red Jujube Pigment on Vascular Endothelial Cell Injury Induced by Hydrogen Peroxide

作　　者：邵佩兰[1] 徐明[2] 郭晓丹[1] 郑安然[1] 周华佩 李宛陶 SHAO Peilan;XU Ming;GUO Xiaodan;ZHENG Anran;ZHOU Huapei;LI Wantao(College of Agriculture Science, Ningxia University, Yinchuan 750021, China;College of Life Science, Ningxia University, Yinchuan 750021, China)

机构地区：[1]宁夏大学农学院,宁夏银川750021 [2]宁夏大学生命科学学院,宁夏银川750021

出　　处：《食品科学》2017年第9期173-178,共6页Food Science

基　　金：国家自然科学基金地区科学基金项目(31260375);宁夏自治区级大学生创新创业训练计划项目(20150123)

摘　　要：探讨红枣色素对H_2O_2诱导人血管内皮细胞(human umbilical vein endothelial cells,HUVECs)损伤的保护作用。体外培养HUVECs,随机分为6组:空白对照组、H_2O_2损伤组、红枣色素低、中、高剂量组(50、150、250 mg/L红枣色素)和阳性对照组(50μmol/L VE),采用显微镜观察细胞形态,四甲基偶氮盐法检测细胞增殖活力,试剂盒法测定乳酸脱氢酶(lactate dehydrogense,LDH)活性,流式细胞仪检测细胞凋亡率和线粒体膜电位水平,透射电子显微镜观察细胞超微结构。结果表明,红枣色素可保护H_2O_2对HUVECs的损伤作用,能明显改善受损细胞形态、超微结构,显著降低细胞增殖抑制率、LDH活性、细胞凋亡率,明显提高线粒体膜电位水平(P<0.05),且存在明显的剂量依赖关系。由此推断,红枣色素对氧化应激诱导血管内皮细胞损伤具有一定的保护作用,其机制可能与其抗氧化、抑制细胞凋亡和维持线粒体膜电位有关。This research was designed to explore the protective effect of Chinese red jujube pigment(CRJP)on human vascular endothelial cell injury induced by oxidative hydrogen in vitro.For this purpose,human vascular endothelial cells were cultured in vitro and randomly divided into6groups:control group,oxidative hydrogen injury group,CRJP low-,medium-and high-dose groups(50,150,250mg/L),and positive control group(50μmol/L VE).Cellular morphology,proliferation viability,LDH activity,apoptosis rate,mitochondrial membrane potential and ultrastructure of all groups were determined by inverted microscope,MTT assay,kit assay,flow cytometry and transmission electron microscope,respectively.The results showed that CRJP treatment at various doses had a protective effect on vascular endothelial cell injury induced by oxidative hydrogen.Cellular morphology and ultrastructure of the CRJP intervention groups were improved obviously along with a significant decrease in proliferation inhibition rate,LDH activity and apoptosis rate and a significant increase in mitochondrial membrane potential(P<0.05)in a dose-dependent manner.These results indicate that Chinese red jujube pigment has a protective effect on human vascular endothelial cell injury induced by oxidative hydrogen in vitro and the underlying mechanism may be related to its antioxidant capacity,and its ability to inhibit apoptosis and maintain mitochondrial membrane potential.

关 键 词：红枣色素 血管内皮细胞 过氧化氢 凋亡 线粒体膜电位 

分 类 号：TS201.2[轻工技术与工程—食品科学]