黄酮醇糖苷与茶树品种适制性关系  

Relationship of Flavonol Glycoside and Processing Suitability of Tea Varieties

作　　者：戴伟东[1] 解东超 吕美玲[2] 谭俊峰[1] 李朋亮[1] 吕海鹏[1] 林智[1] DAI Weidong;XIE Dongchao;Lü Meiling;TAN Junfeng;LI Pengliang;Lü Haipeng;LIN Zhi(Key Laboratory of Tea Biology and Resources Utilization, Ministry of Agriculture, Tea Research Institute,Chinese Academy of Agricultural Sciences, Hangzhou 310008, China;Agilent Technologies (China) Co. Ltd., Beijing 100102, China)

机构地区：[1]农业部茶树生物学与资源利用重点实验室,中国农业科学院茶叶研究所,浙江杭州310008 [2]安捷伦科技(中国)有限公司,北京100102

出　　处：《食品科学》2017年第16期104-109,共6页Food Science

基　　金：国家自然科学基金青年科学基金项目(31500561);中国农业科学院创新工程项目(CAAS-ASTIP-2014-TRICAAS);中国农业科学院茶叶研究所基本科研业务费专项(1610212016008);国家现代农业(茶叶)产业技术体系建设专项(CARS-23)

摘　　要：对不同加工适制性的18个茶树品种春季茶叶中10种黄酮醇糖苷进行了超高效液相色谱-四极杆-飞行时间质谱联用检测,并计算了葡萄糖苷化黄酮醇与半乳糖苷化黄酮醇的比值。结果发现相比于适制绿茶的茶树品种,适制红茶品种茶叶中黄酮醇葡萄糖苷化水平较高,而半乳糖苷化水平较低。山柰酚-3-葡萄糖苷含量、山柰酚-3-葡萄糖酰芸香糖苷含量、山柰酚-3-葡萄糖苷/山柰酚-3-半乳糖苷比值、槲皮素-3-葡萄糖苷/槲皮素-3-半乳糖苷比值、杨梅素-3-葡萄糖苷/杨梅素-3-半乳糖苷比值在不同适制性品种间具有显著性差异(P<0.05)。其中山柰酚-3-葡萄糖苷含量和山柰酚-3-葡萄糖苷/山柰酚-3-半乳糖苷比值差异极显著(P<0.001),对于绿茶适制性品种和红茶适制性品种的判断准确率分别为100%和83%,具有作为指导茶树品种适制性生化指标的可能。The contents of10flavonol glycosides in18tea varieties were determined using ultra high performance liquid chromatography-quadrupole-time of flight mass spectrometry(UHPLC-QTOF-MS).The ratios of flavonol glycoside to flavonol galactoside were also calculated.The results showed that the glycosylation level of flavonol was higher in tea varieties with processing suitability for black tea when compared with those suitable for green tea processing.Kaempferol-3-glucoside,kaempferol-3-glucosylrutinoside,kaempferol-3-glucoside to kaempferol-3-galactoside ratio,quercetin-3-glucoside to quercetin-3-galactoside ratio,myricetin-3-glucoside to myricetin-3-galactoside ratio were significantly different(P<0.05)among tea varieties with different processing suitabilities.The significant difference(P<0.001)in kaempferol-3-glucoside and its ratio to kaempferol-3-galactoside indicates the potential for the discrimination of the processing suitability of tea varieties.The discrimination accuracy of tea varieties for their suitability for processing of green and black tea were100%and83%,respectively.

关 键 词：茶叶 品种适制性 黄酮醇糖苷 葡萄糖苷 半乳糖苷 液相色谱-质谱联用 

分 类 号：S571.1[农业科学—茶叶生产加工]