新疆莎车1号扁桃仁2S-清蛋白的纯化及特性研究  

Purification and Characteristics of 2S-Albumin in Shache No.1 Almond from Xinjiang

作　　者：李述刚[1] 陆健康[2] 王萍[2] 马美湖[3] LI Shugang;LU Jiankang;WANG Ping;MA Meihu(Glyn O. Phillips Hydrocolloid Research Center, Hubei Collaborative Innovation Center for Industrial Fermentation, School of Biological Engineering and Food, Hubei University of Technology, Wuhan 430068, China;Production & Construction Group Key Laboratory of Special Agricultural Products Further Processing in Southern Xinjiang, College of Life Sciences, Tarim University,Alar 843300, China;College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China)

机构地区：[1]湖北工业大学生物工程与食品学院湖北工业发酵协同创新中心菲利普斯亲水胶体研究中心,湖北武汉430068 [2]塔里木大学生命科学学院南疆特色农产品深加工兵团重点实验室,新疆阿拉尔843300 [3]华中农业大学食品科学技术学院,湖北武汉430070

出　　处：《食品科学》2017年第17期36-41,共6页Food Science

基　　金：国家自然科学基金青年科学基金项目(31401644);兵团中青年创新领军人才项目(2016BC001)

摘　　要：清蛋白是扁桃种仁中主要蛋白质,为揭示扁桃仁主要蛋白质组成及结构特性,以新疆莎车1号(SC-1)扁桃仁为研究对象,对其种仁中2S-清蛋白进行分离纯化和结构特性研究,即经有机溶剂多次沉淀提取,获得扁桃仁清蛋白的粗提物,再经Q Sepharose FF分离纯化,获得扁桃仁2S-清蛋白。经高效液相色谱-质谱联用分析表明,纯化后的扁桃仁2S-清蛋白准确分子质量为31.40 k D,其在十二烷基硫酸钠-聚丙烯酰胺凝胶电泳结果中显示为18.0 k D和19.5 k D 2个条带,表明其由两个不同的亚基通过二硫键结合而成。扁桃仁2S-清蛋白中谷氨酸(Glu)、天冬氨酸(Asp)和精氨酸(Arg)的含量最高;其二级结构中主要为β-折叠,占65.9%;扁桃仁2S-清蛋白羰基含量为4.45 nmol/mg、活性巯基含量为39.95?μmol/g、总巯基含量为57.11?μmol/g、表面疏水性为23.45?μg;变性温度为43.3℃。研究结果可为新疆扁桃仁的开发利用提供理论依据。Albumin is the major protein in almond.In this experiment,we purified and structurally characterized2S-albumin from Shache No.1almond(SC-1)from Xinjiang.Crude albumin extract was obtained from almond by repeated precipitation with organic solvents and then fractionated chromatographically on Q Sepharose FF to obtain2S-albumin.By HPLC-MS analysis,the accurate molecular mass of2S-albumin was determined to be31.40kD,which was separated into two bands(18.0and19.5kD)in sodium dodecyl sulfate polyacrylamide gel electrophoresis(SDS-PAGE),indicating that it was composed of two distinct subunits connected through disulfide bonds.In2S-albumin,the contents of glutamic acid,aspartic acid and arginine residues were relatively high,and its dominant secondary structure wasβ-sheet(65.9%).The carbonyl group content of2S-albumin was4.45nmol/mg,the active thiol group content was39.95μmol/g,the total thiol group content was57.11μmol/g,the surface hydrophobicity was23.45μg,and the denaturation temperature was43.3℃.These results can provide a theoretical support for the development and utilization of Xinjiang grown almond.

关 键 词：扁桃仁 2S-清蛋白 纯化 结构特性 

分 类 号：TS255.1[轻工技术与工程—农产品加工及贮藏工程]