不同黑茶提取物功能性成分分析及活性评价  

Functional Components and Activities of Different Dark Tea Extracts

作　　者：曹永[1] 赵谋明[1] 赵甜甜[1] 崔春[1] 赵强忠[1] 冯云子[1] CAO Yong;ZHAO Mouming;ZHAO Tiantian;CUI Chun;ZHAO Qiangzhong;FENG Yunzi(College of Food Science and Engineering, South China University of Technology, Guangzhou 510641, China)

机构地区：[1]华南理工大学食品科学与工程学院,广东广州510641

出　　处：《食品科学》2017年第18期54-59,共6页Food Science

基　　金：国家自然科学基金青年科学基金项目(31501424)

摘　　要：探究不同黑茶提取物(天尖茶、茯砖茶、百两茶、六堡茶及普洱茶)中主要功能性组分的差异,评价其功能性活性1,1-二苯基-2-三硝基苯肼(1,1-diphenyl-2-picrylhydrazyl,DPPH)自由基清除能力、氧自由基的吸收能力(oxygen radical absorbance capacity,ORAC)及对PC12神经细胞保护作用,并建立功能性组分与其活性的相关性。结果表明,湖南产的茯砖、天尖和百两茶,其提取物中的茶多酚、茶多糖及儿茶素总含量均显著高于普洱和六堡茶提取物,而咖啡碱、没食子酸及茶氨酸含量则以普洱茶提取物最高。茯砖、天尖和百两茶提取物对DPPH自由基的清除能力及对氧自由基的吸收能力均较强,而在PC12神经细胞损伤保护实验中,茯砖、普洱及六堡茶提取物效果明显。通过相关性分析,发现茶多酚(特别是酯型儿茶素表儿茶素没食子酸酯(epicatechin gallate,ECG)和表没食子儿茶素没食子酸酯(epigallocatechin gallate,EGCG))是黑茶提取物DPPH自由基和ORAC活性的主要影响因素,而茶氨酸含量则与PC12细胞保护作用正相关。The functional components of different dark tea extracts(Tianjian tea,Fuzhuan tea,Bailiang tea,Liubao tea and Pu-erh tea)were quantified and the functional properties,including1,1-diphenyl-2-picrylhydrazyl(DPPH)radical scavenging ability,oxygen radical absorbance capacity(ORAC),and the protective effect against injury in PC12cells,were evaluated.Then the relationship between functional components and properties in tea extracts was analyzed.The results showed that the contents of tea polyphenols,polysaccharides and catechins in Fuzhuan tea,Tianjian tea and Bailiang tea extracts were significantly higher than those in Pu-erh tea and Liubao tea extracts,while Puer tea extract contained the highest contents of caffine,gallic acid and theanine.Fuzhuan tea,Tianjian tea and Bailiang tea extracts had higher ORAC and DPPH radical scavenging activities,whereas Fuzhuan,Puer and Liubao extracts had stronger protective effects against injury in PC12cells.Correlation analysis indicated that DPPH radical scavenging activity and ORAC activity were significantly influenced by the contents of tea polyphenols,especially the contents of epicatechin gallate(ECG)and epigallocatechin gallate(EGCG),while the protective effects against glutamate-induced injury in PC12cells were significantly related to the content of theanine.

关 键 词：黑茶提取物 功能性成分 DPPH自由基 ORAC PC12细胞 

分 类 号：TS202.3[轻工技术与工程—食品科学]