响应面试验优化酶解法制备海洋微藻微拟球藻抗氧化肽工艺  

Optimization of Enzymatic Preparation of Antioxidant Peptides from Protein Hydrolysate of the Marine Microalgae Nannochloropsis by Response Surface Methodology

作　　者：吕小京 操德群[1,2] 徐年军 Lü Xiaojing;CAO Dequn;XU Nianjun(Key Laboratory of Marine Biotechnology of Zhejiang Province, School of Marine Sciences, Ningbo University, Ningbo 315211,China;Key Laboratory of Applied Marine Biotechnology of Ministry of Education, Ningbo University, Ningbo 315211, China)

机构地区：[1]宁波大学海洋学院,浙江省海洋生物工程重点实验室,浙江宁波315211 [2]宁波大学应用海洋生物技术教育部重点实验室,浙江宁波315211

出　　处：《食品科学》2018年第6期183-188,共6页Food Science

基　　金：宁波市科技局科技富民计划项目(2016C10034);浙江省教育厅科研项目(Y201432453)

摘　　要：以海洋微藻饵料微拟球藻蛋白为原料,采用胃蛋白酶酶解制备微拟球藻抗氧化肽。以酶解产物的水解度和1,1-二苯基-2-三硝基苯肼自由基清除活性为评价指标,研究酶底比(质量百分比)、底物质量浓度、酶解温度和p H值对酶解产物的影响。在单因素试验的基础上,利用响应面法优化制备高抗氧化活性抗氧化肽的工艺条件,确定最佳酶解工艺条件为:底物质量浓度5.0 mg/m L、酶解温度32℃、p H 1.36、酶底比6%。在最佳酶解条件下实际测得微拟球藻抗氧化肽的DPPH自由基清除率为51.85%。Protein from the marine microalga Nannochloropsis was extracted and hydrolyzed with pepsin to prepare antioxidant peptides.The effects of enzyme-to-substrate ratio,substrate concentration,hydrolysis temperature and pH on the degree of hydrolysis(DH)and1,1-diphenyl-2-picrylhydrazyl(DPPH)radical scavenging activity of hydrolysates were investigated and the process conditions were optimized using one-factor-at-a-time(OFAT)method and response surface methodology(RSM).The optimal hydrolysis conditions were determined as follows:substrate concentration,5.0mg/mL;temperature,32℃;pH,1.36;and enzyme-to-substrate ratio,6%.The hydrolysate prepared under these conditions could scavenge51.85%of DPPH free radical.

关 键 词：微拟球藻 抗氧化肽 DPPH自由基清除活性 水解度 响应面法 

分 类 号：TS201.1[轻工技术与工程—食品科学]