海藻酸钠微胶囊对乳酸菌产乙醛脱氢酶在体外胃肠消化环境中保护作用  

Protection of Lactobacillus ALDH activity by sodium alginate microcapsule during gastrointestinal digestion in vitro

作　　者：万茵[1,2] 罗成[1,2] 张鸿婷[1,2,3] 郭帅玲 皮潇文 童火艳 付桂明[1,2,3] WAN Yin;LUO Cheng;ZHANG Hong-ting;GUO Shuai-ling;PI Xiao-wen;TONG Huo-yan;FU Gui-ming(State Key Laboratory of Food Science and Technology,Nanchang University,Nanchang 330047,China;College of Food Science and Technology,Nanchang University,Nanchang 330047,China;Sino-German Food Engineering Center,Nanchang University,Nanchang 330047,China)

机构地区：[1]南昌大学,食品科学与技术国家重点实验室,江西南昌330047 [2]南昌大学食品学院,江西南昌330047 [3]南昌大学中徳食品工程中心,江西南昌330047

出　　处：《食品与发酵工业》2018年第9期61-65,共5页Food and Fermentation Industries

摘　　要：利用海藻酸钠及脱脂奶粉等材料,采用氯化钙挤压法包埋产乙醛脱氢酶的植物乳杆菌Lactobacillus plantarum FCJX 102和嗜酸乳杆菌Lactobacillus acidophilus FCJX 104,并探讨其在体外模拟胃肠液中对乙醛脱氢酶的保护作用。研究结果表明,双乳杆菌海藻酸钠微胶囊平均粒径为1. 14 mm,外表圆润饱满,且包埋率达99. 8%。在体外模拟的胃液环境中,消化90 min微胶囊包埋菌体乙醛脱氢酶活性能保留在95%以上,至消化180 min后乙醛脱氢酶活保留率仍然达73. 4%;而未经包埋的双乳杆菌,其乙醛脱氢酶在30 min时就已完全失去活性;在人工肠液中,双乳杆菌海藻酸钠微胶囊颗粒具有较好的肠溶性,菌体乙醛脱氢酶保留率在消化30、50min仍达97. 8%和86. 5%。In this paper,microcapsules of two Lactobacillus strains producing acetaldehyde dehydrogenase(ALDH),Lactobacillus plantarum and Lactobacillus acidophilus,were prepared using calcium chloride extrusion technique with sodium alginate and skimmed milk powder as encapsulants.The protective effect of microcapsules on acetaldehyde dehydrogenase in artificial gastric and intestinal succus in vitro was examined.The results showed that the average particle size of double-Lactobacillus alginate microcapsules was 1.14 mm,the appearance of which was round and full,and the embedding rate reached 99.8%.The ALDH activity from Lactobacillus microcapsules maintained 95%and 73.4%in the artificial gastric succus system after 90 min and 180 min,respectively.However,the ALDH activity of free bacteria in the artificial gastric succus system decreased to zero after 30 min.In the artificial intestinal succus,caalginate embedded Lactobacillus microcapsules had favorable enteric solubility.The retention rate of the ALDH activity was still 97.8%and 86.5%after digestion for 30 min and 50 min.

关 键 词：植物乳杆菌 嗜酸乳杆菌 微胶囊 乙醛脱氢酶 体外胃肠消化 

分 类 号：TS201.3[轻工技术与工程—食品科学]