海洋低温甾醇酯酶菌株的筛选鉴定及其酶学特性研究  

Screening and identification of a low temperature sterol esterase-producing strain from marine and its enzymatic properties

作　　者：任楠楠[1,2] 王晓辉 迟乃玉[1,2] 张庆芳 REN Nannan;WANG Xiaohui;CHI Naiyu;ZHANG Qingfang(College of Life Science and Biotechnology, Dalian University, Dalian 116622, China;Liaoning Technology of Marine Microbiological Engineering Research Center, Dalian 116622, China)

机构地区：[1]大连大学生命科学与技术学院,辽宁大连116622 [2]辽宁省海洋微生物工程技术研究中心,辽宁大连116622

出　　处：《中国酿造》2019年第1期37-41,共5页China Brewing

基　　金：国家高技术研究发展计划"863"计划项目(2007AA021306)

摘　　要：从大连渤海湾海泥海水样品中分离出一株高产海洋甾醇酯酶的菌株Q-06,对其进行形态观察、生理生化试验和分子生物学(16S r DNA)鉴定,并对其产海洋甾醇酯酶进行酶学特性研究。结果表明,菌株Q-06被鉴定为莓实假单胞菌(Pseudomonas fragi),其最适作用温度和pH值分别为30℃和7.0,该酶在低温下酶活性较高,有一定的耐弱碱性。Ag^+对酶的抑制性较强,Mg^(2+) 、Ni^(2+) 、Ca^(2+) 等对甾醇酯酶的活性有微弱的激活作用,乙二胺四乙酸(EDTA)和十二烷基硫酸钠(SDS)均可以有效地抑制甾醇酯酶活性。A strain Q-06 with high production of marine sterol esterase was isolated from marine mud and water samples in the Bohai Bay sea area of Dalian, and was identified by morphological observation, physiological and biochemical experiments and molecular biology (16S rDNA) identification. Results showed that the strain Q-06 was identified as Pseudomonas fragi. The enzymatic properties of the sterol esterase produced by strain Q-06 were researched. The results showed that the optimum temperature and pH value of the sterol esterase were 30 ℃ and 7.0, respectively. The enzyme activity of sterol esterase was higher at low temperature, and there was a certain resistance to alkalescence. The inhibitory effect of Ag^+ on the sterol esterase was significant, the activation effect of Mg^2+, Ni^2+ and Ca^2+ on sterol esterase was weaker, and the ethylene diamine tetraacetic acid (EDTA) and sodium dodecyl sulfate (SDS) could effectively inhibit the activity of sterol esterase.

关 键 词：甾醇酯酶 菌株筛选 鉴定 酶学性质 

分 类 号：Q556.1[生物学—生物化学]