全二维气相色谱/飞行时间质谱分析传统工艺与机械化工艺老白干原酒的挥发性成分  

Analysis of Volatiles in Laobaigan Base Baijiu Manufactured by Traditional Technology and Mechanized Technology Using Comprehensive Two-Dimensional Gas Chromatography/Time of Flight Mass Spectrometry

作　　者：马雅杰[1,2] 郑福平[1,2] 黄明泉[2] 张煜行[3] 张福艳[3] 孙宝国[1,2] Ma Yajie;Zheng Fuping;Huang Mingquan;Zhang Yuhang;Zhang Fuyan;Sun Baoguo(Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology and Business University, Beijing 100048;Beijing Laboratory of Food Quality and Safety, Beijing Technology and Business University, Beijing 100048;Hebei Hengshui Laobaigan Liquor Group Co. Ltd., Hengshui 053000, Hebei)

机构地区：[1]北京工商大学北京食品营养与人类健康高精尖创新中心,北京100048 [2]北京工商大学食品质量与安全北京实验室,北京100048 [3]河北衡水老白干酿酒(集团)有限公司,河北衡水053000

出　　处：《中国食品学报》2019年第3期266-280,共15页Journal of Chinese Institute Of Food Science and Technology

基　　金：国家重点研发计划项目(2016YFD0400500);国家自然科学基金项目(31301466);北京市教委科技计划重点项目(KZ201410011015)

摘　　要：应用顶空固相微萃取法(HS-SPME)结合全二维气相色谱-飞行时间质谱联机对7种传统工艺老白干原酒与3种机械化工艺老白干原酒中的挥发性成分进行分析。从10种原酒中共检测到322种化合物,其中223种经保留指数定性,95种化合物经标准品比对准确定性。从7种传统工艺原酒中共检出305种共有成分,从3种机械化工艺原酒中共检出294种共有成分,两类工艺原酒中有286种共有成分,其中酯类107种,醇类44种,醛类28种,酮类28种,酸类2种,含氮类5种,含硫类6种,呋喃类13种,缩醛类16种,酚类1种,醚类6种,酸酐类3种,烃类27种。这说明老白干香型原酒中的挥发性成分的种类相对稳定。3种机械化工艺原酒中均缺失丙醛和2,3-戊二酮。使用Fisher比率法(F值)对传统工艺与机械化工艺老白干原酒中共有挥发性成分的相对浓度进行了比较,其中F值大于5的化合物有43个。An effective and reliable method based on comprehensive two-dimensional gas chromatography/time of flight mass spectrometry was established for qualitative analyses of volatile compounds of 7 kinds of base Baijiu of Laobaigan manufactured by traditional technology and 3 kinds of base Baijiu of Laobaigan manufactured by mechanized technology respectively. A total of 322 compounds were tentatively identified by NIST search from ten kinds of Laobaigan base Baijiu. Furthermore, comparison with the retention time of volatile standards was used as a reliable qualitative method to identify volatiles with aid of their retention indices(RIs). Among them, 223 volatiles were identified after the comparison with the RIs and 95 compounds were confirmed by their RIs and mass spectra of the authentic standards.According to statistical comparison, a total of 305 compounds were detected in 7 kinds of the traditional technology base Baijiu and 294 compounds were detected in 3 kinds of the mechanized technology base Baijiu. 286 Volatiles were detected in all samples, including 107 esters, 44 alcohols, 28 aldehydes, 28 ketones, 2 acids, 5 nitrogen-containing compounds, 6 sulfur compounds, 13 furans, 16 acetals, 1 phenols, 6 ethers, 3 anhydrides, and 27 hydrocarbons. This indicated the kinds of volatiles were relatively stable between the Baijiu manufactured by traditional technology and mechanized technology respectively. Only propanal and 2,3-pentanedione couldn’t be found in all mechanized technology base Baijiu. The differences of the volatiles in 10 kinds of base Baijiu were compared by Fisher Ratio and 43 compounds shown Fisher Ratio bigger than 5.

关 键 词：老白干原酒 传统工艺与机械化工艺 全二维气相色谱/飞行时间质谱 固相微萃取法 挥发性成分 

分 类 号：O657.63[理学—分析化学] TS262.3[理学—化学]