解淀粉芽孢杆菌胞外多糖对乳酸菌生长及代谢的调控作用  

Effects of Bacillus amyloliquefaciens exopolysaccharides on proliferation and metabolism of lactic acid bacteria

作　　者：蔡国林 刘逸凡[2] 李晓敏 陆健 CAI Guolin;LIU Yifan;LI Xiaomin;LU Jian(Key Laboratory of Industrial Biotechnology,Ministry of Education (Jiangnan University),Wuxi 214122,China;National Engineering Laboratory for Cereal Fermentation Technology (Jiangnan University),Wuxi 214122,China;School of Biotechnology,Jiangnan University,Wuxi 214122,China)

机构地区：[1]工业生物技术教育部重点实验室(江南大学),江苏无锡214122 [2]粮食发酵工艺与技术国家工程实验室(江南大学),江苏无锡214122 [3]江南大学生物工程学院,江苏无锡214122

出　　处：《食品与发酵工业》2019年第10期16-21,共6页Food and Fermentation Industries

基　　金：国家重点基础研究发展计划(973计划,2013CB733602);高等学校学科创新引智计划(111计划)资助项目(111-2-06);江苏高校优势学科建设工程资助项目

摘　　要：研究解淀粉芽孢杆菌JN4的胞外多糖(exopolysaccharide, EPS-JN4)对乳酸菌生长及代谢的调控作用,有利于阐明EPS-JN4定向增殖乳酸菌的作用机制。采用PCR-变性梯度凝胶电泳技术分析EPS-JN4的定向增殖作用,并考察其对主要乳酸菌生长、主要代谢产物、肠道耐受性和表面疏水性的影响。EPS-JN4可以定向增殖罗伊氏乳杆菌,其对罗伊氏乳杆菌JN125的前期增殖速度较葡萄糖慢,但最高菌浓为葡萄糖的5.25倍,代谢产物中乳酸和醋酸含量则较葡萄糖分别降低了12.2%和32.4%。EPS-JN4增殖的罗伊氏乳杆菌JN125的肠道耐受性和表面疏水性显著提高,对其原因初步分析表明细胞膜蛋白和脂类物质含量提高了40.8%和105.7%,细胞膜组成中的十八碳脂肪酸和不饱和脂肪酸显著提高。EPS-JN4可以作为潜在的益生元,用于调节动物肠道健康。This study aimed to investigate the effects of exopolysaccharides from Bacillus amyloliquefaciens JN4 (EPS-JN4) on the propagation and metabolism of probiotics. The specific proliferative effects of EPS-JN4 were analyzed by PCR-denaturing gradient gel electrophoresis, and their effects on the growth, metabolites, intestinal tolerance, and surface hydrophobicity of lactic acid bacteria were also determined. It was found that EPS-JN4 had a specific proliferative effect on Lactobacillus reuteri . Compared to glucose, the early proliferation rate of L. reuteri JN125 treated with EPS-JN4 was slower, but its highest biomass was 4.25 times higher. In addition, the contents of lactic acid and acetic acid reduced by 12.2% and 32.4%, respectively. The intestinal tolerance and surface hydrophobicity of EPS-JN4-treated L. reuteri JN125 significantly improved, which could be due to the protein and lipid contents in cell membranes increased by 40.8% and 105.7%, respectively. Besides, the proportions of 18 C fatty acids and unsaturated fatty acids in cell membranes significantly increased. In conclusion, EPS-JN4 can be used as a potential prebiotic to regulate the intestinal health of animals.

关 键 词：解淀粉芽孢杆菌 胞外多糖 乳酸菌 益生元 生长 代谢 

分 类 号：TS201.3[轻工技术与工程—食品科学]