Propofol decreases the excitability of cholinergic neurons in mouse basal forebrain via GABAa receptors  

作　　者：Lei Chen Zhi-lai Yang Juan Cheng Ping-ping Zhang Le-sha Zhang Xue-sheng Liu Lie-cheng Wang 

机构地区：[1]Department of Pharmacology and Physiology, School of Basic Medical Sciences, Anhui Medical University, Hefei 230032, China [2]Department of Anesthesiology, First Affiliated Hospital of Anhui Medical University, Hefei 230022, China

出　　处：《Acta Pharmacologica Sinica》2019年第6期755-761,共7页中国药理学报（英文版）

基　　金：National Natural Science Foundation of China (81571293 to LcW;81500949 to Z-IY;81571039 to X-sL);National Century Excellent Talents in University of China (gxbjZD2016034 to L-cW).

摘　　要：Propofol is an intravenous anesthetic that can active y-aminobutyric acid A (GABAa) receptors and generate sedative-hypnotic effects. Propofol has been widely applied clinically to achieve sedation comparable to sleep in humans. The basal forebrain (BF) is a brain region that plays an important role in sleep-wake regulation. Previous studies suggest that propofol affects the sleep-wake circuit via the BF;however, the mechanism remains elusive. In the current study we investigated the effects of propofol on the inherent properties of cholinergic neurons and their ability to convert excitatory inputs into spikes in mouse BF slices using wholecell patch clamp recordings. Bath application of propofol (10μM) significantly elevated the threshold potentials (Vts), decreased the number of spikes in response to a depolarizing current injection, and augmented the inter-spike intervals (ISIs), energy barrier (Vts- Vrs), and absolute refractory periods (ARPs). These effects were eliminated by co-application of a GABAa receptor antagonist picrotoxin (50μM). Altogether, our results reveal that propofol decreases the excitability of cholinergic neurons in mouse BF via GABAa receptors.

关 键 词：PROPOFOL basal FOREBRAIN CHOLINERGIC neurons PICROTOXIN GABAA receptors anesthesia 

分 类 号：R[医药卫生]