条浒苔破壁方法研究  

Study on the Breaking Methods of Cell Wall of Enteromorpha clathrata

作　　者：张中堂 徐倩[1] 林倩如 刘晓攀 赵永慧 王熙[1] 史辰娟 盘赛昆[1,2,3] ZHANG Zhongtang;XU Qian;LIN Qianru;LIU Xiaopan;ZHAO Yonghui;WANG Xi;SHI Chenjuan;PAN Saikun(School of Marine Life and Fisheries,Huaihai Institute of Technology,Lianyungang 222005,China;Jiangsu Key Laboratory of Marine Biotechnology,Huaihai Institute of Technology,Lianyungang 222005,China;Co-innovation Center of Jiangsu Marine Bio-industry Technology,Huaihai Institute of Technology,Lianyungang 222005,China)

机构地区：[1]淮海工学院海洋生命与水产学院,江苏连云港222005 [2]淮海工学院江苏省海洋生物技术重点实验室,江苏连云港222005 [3]淮海工学院江苏省海洋生物产业技术协同创新中心,江苏连云港222005

出　　处：《淮海工学院学报（自然科学版）》2019年第2期89-92,共4页Journal of Huaihai Institute of Technology:Natural Sciences Edition

基　　金：江苏省“六大人才高峰”高层次人才资助项目(NY-197);连云港市第五期“521工程”科研项目;江苏省科技厅科技计划(苏北科技专项)项目(BN2015051);淮海工学院江苏省海洋生物技术重点实验室开放课题(HS16002);淮海工学院研究生实践创新计划项目(XKYCXZ2017-8,XKYCXZ2017-9)

摘　　要：为了建立一种有效的条浒苔细胞壁破壁方法,以显微观察和蛋白质提取率为评价指标,考察干法超微粉碎法、湿法超微粉碎法、超声波粉碎法、湿法超微粉碎+酶解法和超声波粉碎+酶解法5种方法对条浒苔细胞壁的破坏效果.结果表明,5种方法中湿法超微粉碎+酶解法处理后,细胞组织形态被破坏,细胞之间排布零散无规律,细胞内组织液流出,此方法蛋白质提取率最高,可达到30.86%.In order to establish an effective cell wall breaking method for Enteromorpha clathrata,five methods which include dry superfine pulverization, wet ultrafine pulverization, ultrasonic pulverization, ultrasonic pulverization with enzymatic hydrolysis and wet ultrafine pulverization with enzymatic hydrolysis are investigated to evaluate the effect of destruction on the cell wall of Enteromorpha clathrata by using microscopic observation and the protein extraction rate as index.The results demonstrate that the method of wet ultrafine pulverization with enzymatic hydrolysis treatment can breakdown the cell morphous, make the cells scatter irregularly and the enchylema is flowed out.The extraction rate of protein treated by this method can reach 30.86%, which is the highest among the tested methods.

关 键 词：条浒苔 破壁 蛋白质提取率 湿法超微粉碎 酶解法 

分 类 号：TS254.1[轻工技术与工程—水产品加工及贮藏工程]