酪蛋白胰酶水解物的抗氧化活性及稳定性  

Antioxidant activity and stability of casein tryptic hydrolysate

作　　者：陈旭峰 王佳丽[2] 樊伟鑫 许女[2] CHEN Xufeng;WANG Jiali;FAN Weixin;XU Nyu(Center of Experiment and Teaching,Shanxi Agricultural University, Taigu, Shanxi 030801, China;College of Food Science and Engineering,Shanxi Agricultural University, Taigu, Shanxi 030801, China)

机构地区：[1]山西农业大学实验教学中心,山西太谷030801 [2]山西农业大学食品科学与工程学院,山西太谷030801

出　　处：《中国乳品工业》2019年第6期4-9,共6页China Dairy Industry

基　　金：山西省科技重大专项项目(201703D211001-06-05);山西省应用基础研究项目(201701D221189)

摘　　要：本文主要对酪蛋白酶解制备抗氧化肽的工艺进行优化,采用小鼠试验对酪蛋白抗氧化肽的体外、体内抗氧化活性进行了研究,并测试了其耐消化、耐酸碱、耐热及储存稳定性。通过单因素试验,优化得到酪蛋白胰蛋白酶水解的最佳工艺为:底物浓度[S]为50 mg/mL,加酶量[E]/[S]为4%,温度50℃,p H为7.5,水解时间120 min。最优酶解条件下,水解度为30.1%,1,1-二苯基-2-苦基肼基自由基(1,1-Diphenyl-2-picrylhydrazyl,DPPH)自由基清除率为68.1%。此酪蛋白胰蛋白酶水解物(Casein trypsin hydrolysate,CTH)对羟自由基(·OH)和一氧化氮自由基(NO·)也有着较好的清除效果,并且具有浓度相关性,当多肽浓度为10 mg/mL时,对二者的清除率分别达到45.2%和61.5%;另外,其对超氧阴离子自由基(O2^-·)及亚铁离子(Fe^2+)诱发的卵黄脂蛋白脂质过氧化都具有一定的清除和抑制能力。小鼠试验表明,饲养3 d后,相比于模型组,CTH组小鼠的血清、肝、脑中谷胱甘肽过氧化物酶(Glutathione peroxidase,GSH-Px)、超氧化物歧化酶(Superoxide dismutase,SOD)的活性显著升高,而丙二醛(Malonaldehyde,MDA)含量显著降低。稳定性试验证明酪蛋白酶解产物具有良好的耐消化、耐热、耐酸碱和储藏稳定性,体现了其作为抗氧化功能原料的良好应用潜力。In this paper, the process of preparation of antioxidant peptides by casein hydrolysis was optimized, and the antioxidant activity in vitro and in vivo of casein antioxidant peptides was studied by mouse test. Then the resistance to digestion, acid, alkali, heat and storage stability were tested. By single factor test, the optimum enzyme hydrolysis conditions of trypsin were determined as: [S] 50 mg/mL, [E]/[S] 4 %,temperature 50 ℃, pH 7.5, 120 min. The attained degree of hydrolylsis(DH) was 30.1 %, and 1,1-Diphenyl-2-picrylhydrazyl(DPPH) scavenging activity could reach 68.1 %. The good scavenging effect of the casein trypsin hydrolysate(CTH) on · OH and NO· was also proved with 45.2 % and 61.5 %, respectively, when the concentration of polypeptide was 10 mg/mL. Moreover, the CTH had a certain scavenging effect on O2-· and inhibiting effect to lipid-peroxidation. Compared with model group, the glutathione peroxidase(GSH-Px) and superoxide dismutase(SOD) activities of CTH treatment group were significantly raised, and the malonaldehyde(MDA) content was remarkably reduced. Additionally, our results showed that CTH shave good resistance to digestion, acid, alkali, heat and storage. All of this conceived the good potential applications as antioxidant functional raw material.

关 键 词：酪蛋白 水解物 抗氧化活性 稳定性 

分 类 号：TQ931[化学工程]