富硒平菇多糖对益生菌生长代谢的研究  

Effect of selenium-enriched Pleurotus ostreatus polysaccharides on growth and metabolism of probiotics

作　　者：郭芸 李海涛[2] 赵玉明[2] 马玲[3] GUO Yun;LI Haitao;ZHAO Yuming;MA Ling(Department of Food Engineering,Shanxi Pharmaceutical Vocational College,Taiyuan 030031,China;Biology Institute of Shanxi,Taiyuan 030006,China;College of Food Science and Technology,Shanxi Agricultural University,Jinzhong 030801,China)

机构地区：[1]山西药科职业学院食品工程系,山西太原030031 [2]山西省生物研究所,山西太原030006 [3]山西农业大学食品科学与工程学院,山西晋中030801

出　　处：《中国酿造》2019年第11期145-148,共4页China Brewing

基　　金：山西省重点研发计划重点项目(201603D21108-02);山西省重点研发计划项目(201703D211001-06-06);山西农业大学博士科研启动项目

摘　　要：以2%葡萄糖和2%菊粉分别作为对照,测定在无葡萄糖的MRS培养基中添加2%平菇多糖,0.5%和2%富硒平菇多糖对副干酪乳杆菌(Lactobacillus paracasei)和植物乳杆菌(Lactobacillus plantarum)发酵后活菌数、pH及代谢产物的影响。结果显示,发酵24 h后,平菇多糖和富硒平菇多糖都促进了副干酪乳杆菌和植物乳杆菌两种益生菌的增殖,其中0.5%富硒平菇多糖效果更好;2%富硒平菇多糖对发酵48 h副干酪乳杆菌的增殖效果优于菊粉,对植物乳杆菌的增殖效果与菊粉相当。平菇多糖和富硒平菇多糖促进了副干酪乳杆菌和植物乳杆菌代谢生成短链有机酸的含量,其中2%富硒平菇多糖的促进作用更强,两种益生菌发酵后的甲酸、乙酸、丙酸、丁酸含量分别比对照组(葡萄糖2%)提高了340%、32.5%、43.4%、51.4%和122.7%、186.5%、183.3%、412.5%。The effects of Pleurotus ostreatus polysaccharides 2%,selenium-enriched P.ostreatus polysaccharides 0.5%and 2%addition in the MRS medium without glucose on the total viable count of Lactobacillus paracasei and Lactobacillus plantarum,pH and metabolite products were determined,with the addition of glucose 2%and inulin 2%as control,respectively.The results showed that P.ostreatus polysaccharides and selenium-enriched P.ostreatus polysaccharides stimulated the growth of two probiotics of L.paracasei and L.plantarum after fermentation for 24 h,and the treatment of selenium-enriched P.ostreatus polysaccharides 0.5%was better.Selenium-enriched P.ostreatus polysaccharides 2%was better than inulin on stimulating growth of L.paracasei and as the same effect of inulin on stimulating growth of L.plantarum after fermentation for 48 h.P.ostreatus polysaccharides and selenium-enriched P.ostreatus polysaccharides promoted the production of short-chain organic acids by L.paracasei and L.plantarum,and selenium-enriched P.ostreatus polysaccharides 2%had even better effect.Compared with the control(glucose 2%),the content of formic acid,acetic acid,propanoic acid and butyric acid increased 340%,32.5%,43.4%,51.4%and 122.7%,186.5%,183.3%,412.5%after fermentation by L.paracasei and L.plantarum,respectively.

关 键 词：富硒平菇多糖 副干酪乳杆菌 植物乳杆菌 活菌数 短链有机酸 

分 类 号：TS219[轻工技术与工程—粮食、油脂及植物蛋白工程]