二烯丙基二硫醚对产志贺毒性大肠杆菌毒力基因表达及生物学特性的影响  

The Effect of Diallyl Disulfide on the Shiga Toxin-producing Escherichia coli in the Expression of Virulence Gene and Biological Characteristics

作　　者：李鑫鑫 杨春雪 吴虹燕[1] 魏丽娜 侯红漫[1] 张公亮[1] Li Xinxin;Yang Chunxue;Wu Hongyan;Wei Lina;Hou Hangman;Zhang Gongliang(School of Food Seience and Technology,Dalian Polytechnic University,Dalian 116034,Liaoning)

机构地区：[1]大连工业大学食品学院

出　　处：《中国食品学报》2020年第1期76-82,共7页Journal of Chinese Institute Of Food Science and Technology

基　　金：国家自然科学基金项目（31571888）

摘　　要：以二烯丙基二硫醚(DADS)为原料,以非O157产志贺毒性大肠杆菌CICC10670为供试菌,通过测定最低抑菌浓度探究DADS对大肠杆菌的抑菌活性,利用实时荧光定量PCR技术测定大肠杆菌stx2、eaeA和ehxA基因表达的影响,DADS作为抑制剂探究其对大肠杆菌游动能力的影响。结果表明:DADS对大肠杆菌有较好的抑菌能力,其最低抑菌浓度为2 mmol/L。亚抑菌浓度下DADS降低大肠杆菌毒力基因stx2、eaeA和ehxA的表达,相对表达量降低85%以上,并呈现浓度依赖性。DADS以剂量依赖的方式在亚抑菌浓度下抑制大肠杆菌游动能力。综上所述,二烯丙基二硫醚对产志贺毒性大肠杆菌有良好的抑菌作用,并且较低浓度下就可抑制相关毒力基因的表达,可作为潜在的天然防腐剂,防止食物中毒,延长食品的货架期。In the study DADS was selected as material,and the antibacterial activities and the minimal inhibitory concentration of DADS against non-O157 Shiga toxin-producing Escherichia coli CICC10670 were determined.Real-time quantitative reverse transcriptase-polymerase chain reaction was performed to evaluate the effect of DADS on the virulence factors stx2,eaeA and ehxA of E.coli.The effect of DADS as an inhibitor on the motility of Escherichia coli.The result showed that DADS can the higher inhibitory effect against E.coli.Subinhibitory concentrations of DADS inhibited the expression of stx2,eaeA and ehxA.The relative expression decreased to more than 85%,and a dose-dependent attenuation of stx2,eaeA and ehxA production was observed.DADS inhibited the ability of E.coli to swim in a dose-dependent manner at sub MIC concentrations.In summary,DADS has good antibacterial effect on the pathogenic E.coli and low concentration will inhibit expression of the virulence genes,DADS is suitable as a potential natural preservative,preventing food poisoning and extending the shelf life of food.

关 键 词：二烯丙基二硫醚 大肠杆菌 抑菌 毒力因子表达 生物学特性 

分 类 号：TS264.3[轻工技术与工程—发酵工程]