超高效液相色谱法同时检测茶叶中的没食子酸、咖啡碱和儿茶素  

Simultaneous determination of gallic acid,caffeine and catechins in tea by UPLC

作　　者：赵明明[1] 周聪 彭茂民[1] 周有祥[1] ZHAO Ming-ming;ZHOU Cong;PENG Mao-min;ZHOU You-xiang(Institute of Quality Standard and Testing Technology for Agro-Products,Hubei Academy of Agricultural Sciences,Wuhan 430064,China)

机构地区：[1]湖北省农业科学院农业质量标准与检测技术研究所

出　　处：《湖北农业科学》2019年第24期205-208,共4页Hubei Agricultural Sciences

摘　　要：通过优化前处理提取条件和超高效液相色谱分离方法,建立了茶叶中没食子酸、咖啡碱和8种儿茶素定性和定量方法。结果表明,选用ACQUITY UPLC BEH C18(1.7μm,2.1 mm×50 mm)色谱柱,色谱条件为柱温30℃,流速0.2 mL/min,流动相选择0.1%甲酸水-0.1%甲酸甲醇溶液,检测波长278 nm,10种组分在0.05~10.00 mg/L具有良好的线性关系,绿茶样品中各组分含量的相对偏差RSD在1.77%~5.89%,表明该方法简洁快速,且精密度好。A qualitative and quantitative method for gallic acid, CAF and 8 catechins in tea were established by optimizing the extraction conditions of pretreatment and separation methods of Ultra Performance Liquid Chromatography(UPLC). The results showed that the ACQUITY UPLC BEH C18(1.7 μm,2.1 mm×50 mm) chromatographic column was used and the chromatographic conditions were as follows:The column temperature was 30 ℃, the flow rate was 0.2 mL/min, the mobile phase was0.1% formic acid water-0.1% formic acid methanol solution, the detection wavelength was 278 nm. The 10 components in the range of 0.05 ~10.00 mg/L had a good linear relationship, the relative deviation of the content of each component in green tea samples RSD between 1.77%~5.89%. The method is simple, fast and accurate.

关 键 词：茶叶 超高效液相色谱法 没食子酸 咖啡碱 儿茶素 

分 类 号：S571.1[农业科学—茶叶生产加工] O657.72[农业科学—作物学]