响应面法优化黄浆水发酵液制备工艺及其抗氧化活性研究  

Optimization of Preparation of Fermented Tofu Whey by Response Surface Methodology and Their Antioxidant Activity

作　　者：王珊珊[1] 孙晓琦[1] 马玉洁[1] 李娜[1] 周德庆[1,2] WANG Shan-shan;SUN Xiao-qi;MA Yu-jie;LI Na;ZHOU De-qing(Yellow Sea Fishery Research Institute,Chinese Academy of Fishery Sciences,Qingdao 266071,Shandong,China;Laboratory of Marine Drugs and Biological Products,Pilot National Laboratory for Marine Science and Technology,Qingdao 266000,Shandong,China)

机构地区：[1]中国水产科学研究院黄海水产研究所,山东青岛266071 [2]青岛海洋科学与技术试点国家实验室海洋药物与生物制品功能实验室,山东青岛266000

出　　处：《食品研究与开发》2020年第4期45-51,共7页Food Research and Development

基　　金：中国水产科学研究院基本科研业务费资助（2019ZD1002）;山东省农业重大应用技术创新课题（2016)

摘　　要：采用植物乳杆菌(Lactobacillus plantarum)、嗜酸乳杆菌(Lactobacillus acidophilus)和清酒乳杆菌(Lactobacillus sakei)3种乳酸菌对黄浆水进行组合发酵,以发酵液DPPH自由基清除率为评价指标,研究发酵温度、发酵时间、脱脂乳粉添加量、葡萄糖添加量和接种量对发酵液的影响。在单因素试验基础上,采用响应面法优化制备高抗氧化活性黄浆水发酵液的工艺条件。结果表明最佳发酵参数为:接种量1%、发酵温度37℃、发酵时间37.50 h、脱脂乳粉添加量8%、葡萄糖添加量5%。在此条件下制备的黄浆水发酵液DPPH自由基清除率为82.36%。抗氧化活性试验表明:黄浆水发酵液提取物抗氧化能力得到显著提升,其清除DPPH自由基、羟自由基和ABTS自由基的半抑制质量浓度(IC50)分别为2.03 mg/mL、1.12 mg/mL和0.30 mg/mL。The purpose of this study was to investigate the optimal fermentation conditions(fermentation temperature,fermentation time,skim milk content,glucose content and inoculum amount)on the DPPH scavenging rate of fermented tofu whey using Lactobacillus plantarum,Lactobacillus acidophilus and Lactobacillus sakei.On the basis of single factor test,the response surface methodology(RSM)was used to optimize the preparation of fermented tofu whey with high antioxidant activity.The results showed that the optimal fermentation conditions were inoculum amount of 1%,fermentation temperature of 37℃,fermentation time of 37.50 h,skimmed milk content of 8%and glucose content of 5%.The DPPH scavenging rate under the optimal conditions was 82.36%.The antioxidant activity test showed that the antioxidant capacity of the fermented tofu whey extracts was significantly improved with half inhibitory concentration(IC50)for scavenging DPPH,hydroxyl and ABTS radicals of 2.03 mg/mL,1.12 mg/mL and 0.30 mg/mL,respectively.

关 键 词：植物乳杆菌 嗜酸乳杆菌 清酒乳杆菌 黄浆水 发酵工艺 响应面分析 抗氧化活性 

分 类 号：X792[环境科学与工程—环境工程] TS275[轻工技术与工程—农产品加工及贮藏工程]