中草药复配乳蛋白ACE抑制肽的试验研究  

Experimental Study on Chinese Herbal Medicine Compounding Milk Protein ACE Inhibitory Peptide

作　　者：赵敏 王志耕[1] ZHAO Min;WANG Zhi-geng(College of Tea and Food Science and Technology,Anhui Agricultural Univercity,Hefei 230036,China)

机构地区：[1]安徽农业大学茶与食品科技学院

出　　处：《食品工业科技》2020年第5期151-155,162,共6页Science and Technology of Food Industry

摘　　要：为了将具有降血压功效的药食同源中草药与具有降血压活性的牛奶蛋白酶解液复配,以完成一款具有辅助降压功效的功能性牛奶的实验研究提供理论依据和技术参数。试验以ACE抑制率为指标,优化胰蛋白酶酶解脱脂乳的最佳工艺,并从15味中草药中筛选出具有较高ACE抑制活性的提取液,再将优化所得酶解液和筛选出的中草药提取液进行复配,获得高ACE抑制率的活性功能乳组方。结果表明:胰蛋白酶酶解脱脂乳的最佳酶解工艺为底物质量浓度7 g/100 mL、酶解时间6 h、加酶量5%、酶解温度31℃、pH6.9,脱脂乳酶解液的ACE抑制率为58.51%;筛选得出山楂、决明子的ACE抑制率较高,分别为50.88%、45.34%;且当山楂提取液和决明子提取液混合时ACE抑制活性提高,两者为2∶1时,ACE抑制率较高为52.52%;酶解液和中草药提取液(山楂提取液∶决明子提取液为2∶1)按1∶1进行复配,ACE抑制率最高为59.09%,对研究具有辅助调节血压效应的保健功能性乳制品具有理论和实践探讨意义。To provide theoretical basis and technical parameters for the experimental study of a functional milk with auxiliary blood pressure,in this study,the medicine-food homologous Chinese herbal medicine with blood pressure lowering effect and milk proteolytic solution with blood pressure lowering activity were compounded. The experiment is to optimize the optimal process of trypsin enzymatically skimmed milk with ACE inhibition rate as an indicator.The extracts with high ACE inhibitory activity were screened from 15 kinds of Chinese herbal medicines.Formula of high ACE inhibitory activity were obtained by mixed milk protein hydrolysate and the selected Chinese herbal extracts. The best reaction conditions were as follows:The substrate concentration 7 g/100 mL,enzymolysis time 6 h,the amount of enzyme 5%,enzymatic hydrolysis temperature 31 ℃,pH6.9,under the experimental conditions,defatted milk solution ACE inhibition rate was 58.51%. Hawthorn and Cassia seed have higher ACE inhibition rates,which are 50.88% and 45.34%,respectively.When Hawthorn and cassia extract were mixed,the ACE inhibitory activity was increasing. The ACE inhibitory activity was 52.52% when Hawthorn and cassia extract were mixed by 2∶1. The ACE inhibitory activity was 59.09% when the enzyme solution and Chinese herbal medicine extract(Hawthorn and cassia mixed extract were mixed by 2∶1)were mixed by 1∶1.It has theoretical and practical significance for the study of health-care functional dairy products with the effect of regulating blood pressure.

关 键 词：脱脂乳 肽 ACE抑制 中草药 功能乳 

分 类 号：TS201.2[轻工技术与工程—食品科学]