Characterization of myelin oligodendrocyte glycoprotein (MOG)35–55- specific CD8^+ T cells in experimental autoimmune encephalomyelitis  

作　　者：Yong Peng Fei-Zhou Zhu Zhi-Xing Chen Jian-Xiong Zhou Lu Gan Shan-Shan Yang Shuai Gao Qian-Qian Liu 

机构地区：[1]Department of Neurology,Affiliated First Hospital of Hunan Traditional Chinese Medical College,Zhuzhou,Hunan 412000,China [2]Department of Biochemistry and Molecular Biology,School of Life Sciences,Central South University,Changsha,Hunan 410013,China

出　　处：《Chinese Medical Journal》2019年第24期2934-2940,共7页中华医学杂志（英文版）

基　　金：This work is supported by the grants from the Natural Science Foundation of Hunan Province,China(No.2018JJ6043);the Health and Family Plans commission of Hunan Province,China(No.B20180815);the Science and Technology Plan Project of Zhuzhou City,Hunan Province,China(No.20160104).

摘　　要：Background:The pathogenesis of multiple sclerosis(MS)is mediated primarily by T cells,but most studies of MS and its animal model,experimental autoimmune encephalomyelitis(EAE),have focused on CD4^+ T cells.The aims of the current study were to determine the pathological interrelationship between CD4 and CD8 autoreactive T cells in MS/EAE.Methods:Female C57BL/6 mice(n=20)were induced by myelin oligodendrocyte glycoprotein(MOG)35-55 peptide.At 14 days after immunization,T cells were isolated from the spleen and purified as CD4^+ and CD8^+ T cells by using CD4 and CD8 isolation kits,and then the purity was determined by flow cytometric analysis.These cells were stimulated by MOG35–55 peptide and applied to proliferation assays.The interferon-gamma(IFN-γ)and interleukin(IL)-4 secretion of supernatant of cultured CD4^+ and CD8^+ T cells were measured by enzyme-linked immunosorbent assays(ELISA).For adoptive transfer,recipient mice were injected with MOG35–55-specific CD8^+ or CD4^+ T cells.EAE clinical course was measured by EAE score at 0–5 scale and spinal cord was examined by staining with hematoxylin and eosin and Luxol fast blue staining.Results:CD8^+ CD3^+ and CD4^+ CD3^+ cells were 86%and 94%pure of total CD3^+ cells after CD8/CD4 bead enrichment,respectively.These cells were stimulated by MOG35–55 peptide and applied to proliferation assays.Although the CD8^+ T cells had a generally lower response to MOG35–55 than CD4^+ T cells,the response of CD8^+ T cells was not always dependent on CD4.CD8^+ T cell secreted less IFN-γand IL-4 compared with CD4^+ T cells.EAE was induced in wildtype B6 na?ve mice by adoptive transfer of MOG35–55-specific T cells from B6 active-induced EAE(aEAE)mice.A similar EAE score and slight inflammation and demyelination were found in naive B6 mice after transferring of CD8^+ T cells from immunized B6 mice compared with transfer of CD4^+ T cells.Conclusion:Our data suggest that CD8^+ autoreactive T cells in EAE have a lower encephalitogenic function but are unique and inde

关 键 词：Autoimmune CD8-Positive T-LYMPHOCYTES ENCEPHALOMYELITIS Experimental Multiple SCLEROSIS Myelin-Oligodendrocyte GLYCOPROTEIN 

分 类 号：R73[医药卫生—肿瘤]