Involvement of moesin phosphorylation in ischemia/reperfusion induced inner blood-retinal barrier dysfunction  

作　　者：Jing Xu Qiong Liu Ming Ma Lin-Jiang Chen Jian Yu Ke Xiong Jing Wu 

机构地区：[1]Department of Ophthalmology,Nanfang Hospital,Southern Medical University,Guangzhou 510515,Guangdong Province,China [2]Huiqiao Medical Center,Nanfang Hospital,Southern Medical University,Guangzhou 510515,Guangdong Province,China

出　　处：《International Journal of Ophthalmology(English edition)》2020年第4期545-551,共7页国际眼科杂志（英文版）

基　　金：Supported by the Science and Technology Planning Project of Guangdong Province(No.201607010386);the Science and Technology Planning Project of Guangzhou(No.201504290959196)。

摘　　要：AIM: To investigate the role of moesin and its underlying signal transduction in retinal vascular damage induced by retinal ischemia-reperfusion(RIR) insult.METHODS: C57 BL/6 mice were subjected to continued ischemia for 45 min, followed by blood reperfusion. The expression and phosphorylation of moesin in retinal vessels were detected by immunohistochemistry and Western blotting. The inner blood-retinal barrier was evaluated using FITCdextran leakage assay on whole-mount retina. Further studies were conducted to explore the effects of p38 mitogen-activated protein kinase(MAPK) pathway on the involvement of moesin in RIR-evoked retinal vascular hyperpermeability response. RESULTS: It revealed that RIR induced moesin phosphorylation in a time-dependent manner after reperfusion. The phosphorylation of moesin was alleviated by inhibitions of p38 MAPK, while this treatment also ameliorated the dysfunction of inner blood-retinal barrier. CONCLUSION: The results suggest that moesin is involved in RIR-evoked retinal vascular endothelial dysfunction and the phosphorylation of moesin is triggered via p38 MAPK activation.

关 键 词：RETINAL ISCHEMIA-REPERFUSION MOESIN p38 MITOGEN-ACTIVATED protein kinase INNER blood-retinal barrier mice 

分 类 号：R774[医药卫生—眼科]