Targeted bile acids and gut microbiome profiles reveal the hepatoprotective effect of WZ tablet(Schisandra sphenanthera extract) against LCA-induced cholestasis  被引量:14

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作  者:LI Dong-Shun HUANG Quan-Fei GUAN Li-Huan ZHANG Hui-Zhen LI Xi FU Kai-Li CHEN Yi-Xin WAN Jian-Bo HUANG Min BI Hui-Chang 

机构地区:[1]Guangdong Provincial Key Laboratory of New Drug Design and Evaluation,School of Pharmaceutical Sciences,Sun Yat-Sen University,Guangzhou 510000,China [2]State Key Laboratory of Quality Research in Chinese Medicine,Institute of Chinese Medical Sciences,University of Macao,Macao 999078,China

出  处:《Chinese Journal of Natural Medicines》2020年第3期211-218,共8页中国天然药物(英文版)

基  金:supported by the Natural Science Foundation of China(Nos.81973392,81573489);the National Key Research and Development Program(No.2017YFE 0109900);the Natural Science Foundation of Guangdong(No.2017A030311018);the 111 project(No.B16047);the Key Laboratory Foundation of Guangdong Province(No.2017B030314030);the Local Innovative and Research Teams Project of Guangdong Pearl River Talents Program(No.2017BT01Y093);the National Engineering and Technology Research Center for New drug Druggability Evaluation(Seed Program of Guangdong Province,No.2017B090903004)。

摘  要:Cholestasis is caused by the obstacle of bile formation or secretion and can develop into severe liver diseases. We previously reported the ethanol extract of Schisandra sphenanthera(Wuzhi tablet, WZ) can significantly protect against lithocholic acid(LCA)-induced intrahepatic cholestasis in mice, partially due to the activation of PXR pathway and promotion of liver regeneration.However, the effect of WZ on the bile acids profile and gut microbiome in cholestastic mice remain unknown. In this study, the effect of WZ against LCA-induced liver injury was evaluated and its effect on the bile acids metabolome and gut microbiome profiles in cholestastic mice was further investigated. Targeted metabolomics analysis was performed to examine the change of bile acids in the serum, liver, intestine and feces. The change of intestinal flora were detected by the genomics method. Targeted metabolomics analysis revealed that WZ enhanced the excretion of bile acids from serum and liver to intestine and feces. Genomics analysis of gut microbiome showed that WZ can reverse LCA-induced gut microbiome disorder to the normal level. In conclusion, WZ protects against LCAinduced cholestastic liver injury by reversing abnormal bile acids profiles and alteration of gut microbiome.

关 键 词:CHOLESTASIS Wuzhi TABLET SCHISANDRA sphenanthera METABOLOME GUT MICROBIOME Bile acids 

分 类 号:R965[医药卫生—药理学]

 

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