低共熔溶液提取铁观音茶多酚工艺的响应面法优化  

Deep Eutectic Solvents Extraction of Polyphenols from Tieguanyin Tea Optimized by Response Surface Method

作　　者：阮怿航 吴亮宇[1] 鲁静[2] 黄秀红 刘丽辰 林金科[1,2] RUAN Yi-hang;WU Liang-yu;LU Jing;HUANG Xiu-hong;LIU Li-chen;LIN Jin-ke(College of Horticulture,Fujian Agriculture and Forest University,Fuzhou,Fujian 350002,China;College of Anxi Tea Science,Fujian Agriculture and Forest University,Quanzhou,Fujian 362000,China)

机构地区：[1]福建农林大学园艺学院,福建福州350002 [2]福建农林大学安溪茶学院,福建泉州362000

出　　处：《福建农业学报》2020年第2期217-225,共9页Fujian Journal of Agricultural Sciences

基　　金：福建省高校产学合作项目(2016N5001);安溪县科技项目(KH1500790);福建省安溪县现代农业产业园协同创新中心项目(KMD18003A)。

摘　　要：【目的】探索一种绿色、环保、安全、高效的铁观音茶多酚提取新方法,为茶多酚绿色提取提供新的技术参考。【方法】以铁观音成品茶为原料,采用新型绿色溶剂--低共熔溶液提取茶多酚;首先筛选出最优的低共熔溶液提取体系,然后在单因素试验的基础上,通过响应面法优化,研究时间、温度、溶液含水率对提取率的影响,得出最佳提取条件并分析其主要成分;最后通过测定DPPH自由基清除率分析茶多酚的抗氧化能力。【结果】筛选出最适的茶多酚低共熔溶液提取体系为:乳酸-甜菜碱,其次得到最佳单因素提取条件为提取时间40 min、提取温度60℃、含水率30%、摩尔比2∶1、固液比1∶40(g:mL);响应面优化分析得到最佳提取条件为:时间46.79min、温度62.48℃、溶液含水率32.15%,在此条件下茶多酚提取率为15.42%。通过高效液相色谱分析茶多酚组分,其中没食子酸含量占1.40%,儿茶素类物质占84.99%,其他组分占13.61%。低共熔溶液法提取的茶多酚DPPH自由基半清除浓度(IC50)值73.89μg·mL-1,比抗坏血酸提高了37.80%。【结论】采用响应面法优化得出的低共熔溶液提取茶多酚最佳工艺条件,可有效提高铁观音茶多酚提取率。【Objective】 A green, environmentally friendly, safe and efficient process to extract polyphenols from tea was explored and optimized using the response surface method.【Methods】 Tieguanyin tea was used for the extraction with deep eutectic solvents(DESs). After the DES was selected and a single factor experiment conducted, the time, temperature and water content in solvent were optimized for the process with respect of polyphenol extraction rate by a response surface experimentation. Antioxidant capacity of the extract was determined according to the DPPH radical scavenging rate.【Results】 Lactic acid and betaine of a molar ratio of 2∶1 with a water content of 30% and a substrate-to-solvent ratio of1∶40(g∶mL) were chosen for the extraction at 60 o C for 40 min. The response surface method optimized the process to apply a moisture content of 32.15% of DESs for a 46.79 min-extraction at 62.48℃ with a yield of polyphenols at 15.42%. The polyphenol extract was analyzed by HPLC to show a gallic acid content of 1.40%, catechins of 84.99%, and the remainders of13.61%. The semi-scavenging concentration(IC50) of the extract was 73.89 μg·mL-1, which was 37.80% higher than that of ascorbic acid.【Conclusion】 The newly developed DESs extraction significantly improved the polyphenol extraction rate from Tieguanyin tea over the conventional process.

关 键 词：低共熔溶液 铁观音 茶多酚 抗氧化活性 成分分析 

分 类 号：TS201.2[轻工技术与工程—食品科学]