沙棘籽粕多酚提取工艺优化、组分分析及抗氧化性能研究  

Optimization of extraction process,component analysis and antioxidant activities of polyphenols from seabuckthorn(Hippophae rhamnoides L.)seed meal

作　　者：孔宇[1] 张倚菲 韩鹏云 韦柳伊 李文华 王禹鹤 赵紫芙 李欣忆 李超[1] KONG Yu;ZHANG Yifei;HAN Pengyun;WEI Liuyi;LI Wenhua;WANG Yuhe;ZHAO Zifu;LI Xinyi;LI Chao(College of Food Engineering and Biotechnology,Tianjin University of Science and Technology,Tianjin 300457,China)

机构地区：[1]天津科技大学食品工程与生物技术学院,天津300457

出　　处：《中国油脂》2020年第4期109-114,共6页China Oils and Fats

基　　金：天津科技大学大学生创新创业训练计划项目(201810057192);天津科技大学大学生实验室创新基金(1814A208)。

摘　　要：为提高沙棘籽粕中蛋白质和多酚的利用率,在确保蛋白质溶出率低的情况下,优化了沙棘籽粕多酚的提取工艺条件。同时,采用HPLC对沙棘籽粕多酚提取液的组分进行了分析,并研究了沙棘籽粕多酚的抗氧化性。结果表明,沙棘籽粕多酚提取的最佳工艺条件为:80%甲醇溶液为提取溶剂,超声提取方式,超声频率40 kHz,超声功率150 W,料液比1∶25,提取温度20℃,提取时间20 min。在最佳提取条件下,多酚得率为(7.83±0.03)%。沙棘籽粕多酚提取液共检出没食子酸、儿茶素、芦丁、杨梅素、槲皮素、山奈酚6种多酚类物质,其中芦丁含量最高,为(1.623±0.224)mg/g(以沙棘籽粕质量计)。沙棘籽粕多酚具有较强的抗氧化性,对DPPH自由基和羟自由基均具有显著清除能力,半数抑制浓度(IC50)分别为0.057、0.147 mg/mL。In order to improve the utilization rate of protein and polyphenols in seabuckthorn(Hippophae rhamnoides L.)seed meal,the extraction process of polyphenols from seabuckthorn(Hippophae rhamnoides L.)seed meal was optimized under the conditions of low protein dissolution rate.At the same time,the components of polyphenols extracted from seabuckthorn(Hippophae rhamnoides L.)seed meal were analyzed by HPLC,and the antioxidant activity of polyphenols was studied.The results showed that the optimal extraction conditions were obtained as follows:80%methanol solution used as the extraction solvent,ultrasonic extraction method,ultrasonic frequency 40 kHz,ultrasonic power 150 W,ratio of material to liquid 1∶25,extraction temperature 20℃,extraction time 20 min.Under the optimal extraction conditions,the yield of polyphenols was(7.83±0.03)%.Six polyphenols were detected in the polyphenol extract of seabuckthorn(Hippophae rhamnoides L.)seed meal,including gallic acid,catechin,rutin,myricetin,quercetin and kaempferol.The content of rutin was the highest,which was(1.623±0.224)mg/g.The polyphenols had significant scavenging ability on DPPH free radical and hydroxyl free radical,and its IC50 were 0.057 mg/mL and 0.147 mg/mL,respectively,which showed that the polyphenols had strong antioxidant activity.

关 键 词：沙棘籽粕 多酚 提取工艺 组分分析 抗氧化性 

分 类 号：TS229[轻工技术与工程—粮食、油脂及植物蛋白工程] TS201.2[轻工技术与工程—食品科学与工程]