白鲢鱼糜漂洗水蛋白质回收工艺研究  

Study on Protein Recovery Process of Silver Carp Surimi Rinsing Water

作　　者：周俊鹏 章蔚 刘茹[3] 朱旭萌 熊光权[1] 汪兰[1] 吴文锦[1] 石柳 ZHOU Jun-peng;ZHANG Wei;LIU Ru;ZHU Xu-meng;XIONG Guang-quan;WANG Lan;WU Wen-jin;SHI Liu(Institute for Farm Products Processing and Nuclear-Agricultural Technology,Farm Produets Processing Research Sub-center of Hubei Innovation Center of Agriculnure Seience and Technology,Hubei Academy of Agricul tural Science,w uhan 430064,China;School of Food and Biologieal Engineering,Hubei University of Teehnology,W uhan 430068,China;College of Food Science and Technology,Huazhong Agriculural University,Wuban 430070,China;Colege of Life Sciences,Yangtze University,Jingzhou 434025,China)

机构地区：[1]湖北省农业科学院农产品加工与核农技术研究所,湖北省农业科技创新中心农产品加工研究分中心,湖北武汉430064 [2]湖北工业大学生物工程与食品学院,湖北武汉430068 [3]华中农业大学食品科技学院,湖北武汉430070 [4]长江大学生命科学学院,湖北荆州434025

出　　处：《食品工业科技》2020年第12期56-61,共6页Science and Technology of Food Industry

基　　金：湖北省技术创新专项重大项目(2017ABA141);湖北省技术创新专项重大项目(2018ABA100)。

摘　　要：以白鲢鱼糜漂洗水为研究对象,采用不同沉降时间、环境温度、种类的酸、pH、絮凝剂和及其添加量对鱼糜漂洗水进行处理,研究其对蛋白质回收率、上清液透光率和絮凝蛋白质的聚丙烯酰胺凝胶电泳(SDS-PAGE)的影响。结果表明,4℃静置60 min有利于漂洗水的沉降;用盐酸调节pH至5.5时,蛋白质回收率和上清液透光率分别为65.8%和50.1%,且与乙酸和柠檬酸相比,盐酸沉降效果更好;经0.6 mg/mL壳聚糖进一步处理,可使漂洗水蛋白质回收率和上清液透光率分别上升至85.8%与87.8%,蛋白质回收效果加强;SDS-PAGE电泳结果表明,漂洗水中蛋白质分子量主要分布在35、42、48、63、100和190 kDa附近;经系列条件处理后(除190 kDa外),各蛋白质含量逐渐增加,且在25.0~28.0 kDa附近产生多条新的蛋白条带。综合各试验指标结果,在4℃环境温度、盐酸调节pH至5.5,再使用0.6 mg/mL壳聚糖处理鱼糜漂洗水60 min,可高效回收白鲢鱼糜漂洗水中的蛋白质。本试验可为工业上鱼糜漂洗水中蛋白质回收和废水净化提供一定理论依据及实际指导。Rinsing water from the production of sliver carp surimi was applied as the research object. The effect of different settling time,temperature,acid species,pH,flocculant species and their addition on the supernatant permeability,protein recovery yield and protein polyacrylamide gel electrophoresis(SDS-PAGE)of recovered proteins from surimi rinsing water were investigated. The results showed that,when adjusted the pH to 5.5 with hydrochloric acid prior to settlement,settlement at 4 ℃ for 60 min was benefit for protein recovery from rinsing water. The protein recovery yield and light transmittance of the supernatant of rinsing water were increased to by 65.8% and 50.1%,respectively,when adjusted the pH to 5.5 with hydrochloric acid prior to settlement,and the values were higher than those of acetic acid and citric acid. Furthermore,protein recovery yield and light transmittance of the supernatant of rinsing water were increased to 85.8% and 87.8%,respectively,with the presence of 0.6 mg/mL chitosan. The SDS-PAGE results showed that the molecular weight of the recovered protein from surimi rinsing water was mainly distributed at 35,42,48,63,100,and 190 kDa. After a series of treatments,the protein content gradually increased(except for the band at 190 kDa),and multiple new protein bands were generated around 25.0~28.0 kDa. Accordingly,the appropriate conditions for protein recovery from the surimi rinse water were that the pH was adjusted to 5.5 with hydrochloric acid,0.6 mg/mL chitosan was added,following by settlement at 4 ℃ for 60 min. This research could provide theoretical basis and practical guidance for protein recovery and waste water purification from surimi rinsing water industrially.

关 键 词：白鲢 鱼糜漂洗水 回收率 透光率 SDS-PAGE 

分 类 号：TS254.0[轻工技术与工程—水产品加工及贮藏工程]