培养条件对副溶血性弧菌AI-2活性及其合成关键基因表达水平的影响  

Effects of different culture conditions on AI-2 activities and expression levels of the critical genes involved in AI-2 synthesis in Vibrio parahaemolyticus

作　　者：林才云 王联珠[1] 许加超[2] 姚琳[1] 曲梦 李风铃[1] 江艳华[1] LIN Cai-Yun;WANG Lian-Zhu;XU Jia-Chao;YAO Lin;QU Meng;LI Feng-Ling;JIANG Yan-Hua(Key Laboratory of Testing and Evaluation for Aquatic Product Safety and Quality,Ministry of Agriculture and Rural Affairs,Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Qingdao 266071,China;College of Food Scinence and Engineering,Ocean University of China,Qingdao 266003,China)

机构地区：[1]中国水产科学研究院黄海水产研究所,农业农村部水产品质量安全检测与评价重点实验室,青岛266071 [2]中国海洋大学食品科学与工程学院,青岛266003

出　　处：《食品安全质量检测学报》2020年第9期2924-2929,共6页Journal of Food Safety and Quality

基　　金：国家自然科学基金项目(31601566);中国水产科学研究院黄海水产研究所基本科研业务费项目(20603022020004);中国水产科学研究院基本科研业务费项目(2020TD71)。

摘　　要：目的以海产品中分离的一株高产信号分子AI-2(Autoinducer-2)的副溶血性弧菌Vp2009027为研究对象,分析不同的培养条件(时间、温度、糖源、pH和盐度)对菌株AI-2活性及其合成关键基因表达水平的影响。方法采用报告菌株检测AI-2的活性变化,采用逆转录-实时荧光定量PCR法检测合成关键基因luxS和pfs表达量的变化。结果菌株培养12 h后AI-2活性达到最高,当培养温度为30℃时AI-2活性最高,添加糖源、中性和偏碱性环境以及适度的盐度均可促进AI-2活性的提高;不同培养条件下AI-2合成关键基因luxS和pfs的表达量均有不同程度的变化,温度和糖源对AI-2活性的影响与对luxS和pfs基因表达量的影响呈正相关,其他条件下AI-2的活性与luxS和pfs的表达量没有明显相关性。结论不同培养条件可以调控AI-2的合成,可以通过改变培养条件来获得不同产量的AI-2。Objective Taking Vibrio parahaemolyticus Vp2009027,a strain with high-intensity signal molecule autoinducer-2(AI-2) isolated from seafood as the research object,to study the effects of culture conditions(time,temperature,sugar source,pH and salinity) on the activity of AI-2 and the expression level of the critical genes involved in synthesis of AI-2.Methods The activity of AI-2 was assayed using the reporter strain and the expression level of the critical genes luxS and pfs was determined by reverse transcription-realtime quantitative PCR.Results The AI-2 activity reached the highest after 12 h cultivation,and the AI-2 activity was the highest when the cultivation temperature was 30℃.Sugar supplemented in media,the neutral and alkaline condition,and moderate salinity could increase the activity of AI-2.Under different culture conditions,the expression levels of the key genes of AI-2 synthesis,luxS and pfs,had varying degrees of change.The influence of temperature and glycogen on the activity of AI-2 was positively correlated with the effect on the expression of luxS and pfs genes.Under other conditions,the activity of AI-2 had no significant correlation with the expression of luxS and pfs Conclusion Different culture conditions can regulate the synthesis of AI-2,and different production of AI-2 can be obtained by changing the culture conditions.

关 键 词：副溶血性弧菌 信号分子AI-2 LUXS PFS 

分 类 号：TS201.3[轻工技术与工程—食品科学]