Alcalase蛋白酶酶解大豆蛋白制备啤酒糖浆复配液的研究  

Preparation of beer syrup complex solution by Alcalase protease enzymatic hydrolysis of soybean protein

作　　者：罗建勇 吴晨孛 黄隽光 周泓江 钱芳 郭峰 黄立新 LUO Jianyong;WU Chenbei;HUANG Junguang;ZHOU Hongjiang;QIAN Fang;GUO Feng;HUANG Lixin(Guangzhou Shuangqiao Company Ltd.,Guangzhou 510280,China;College of Food Science and Engineering,South China University of Technology,Guangzhou 510640,China)

机构地区：[1]广州双桥股份有限公司,广州510280 [2]华南理工大学食品科学与工程学院,广州510640

出　　处：《中国油脂》2020年第8期103-108,共6页China Oils and Fats

基　　金：2017年绿色制造系统集成项目(工信部节函[2017]327号);2019年华南理工大学国家级大学生创新训练计划项目(201910561127);华南理工大学2019年学生研究计划项目(X201910561588)。

摘　　要：采用Alcalase蛋白酶酶解大豆蛋白,通过测定酶解产物的蛋白质回收率、水解度、隆丁(Lundin)区分以及SDS-PAGE电泳图谱分析,确定在酶解条件为酶解温度55℃、底物质量分数5%、酶量750 U/g、pH 8.0、反应时间0.5~1.0 h时所得的酶解液为浅棕色,其Lundin分布较为接近麦汁Lundin区分分布的要求,蛋白质回收率约65%,可与啤酒糖浆复配作为啤酒发酵的氮源。Alcalase protease was used for enzymatic hydrolysis of soybean protein, and the protein recovery rate, hydrolysis degree, Lundin fraction and SDS-PAGE electropherogram of the enzymolysis product were determined. The enzymatic hydrolysis conditions were obtained as follows: enzymolysis temperature 55℃, substrate mass concentration 5%, enzyme dosage 750 U/g, pH 8.0, reaction time 0.5-1.0 h. Under these conditions, the obtained enzymatic hydrolysate was light brown, and its Lundin fraction was closer to the requirement of the distribution of protein in wort. The protein recovery rate was about 65%, which could be combined with beer syrup as a nitrogen source suitable for beer fermentation.

关 键 词：啤酒糖浆 大豆蛋白 ALCALASE蛋白酶 酶解 隆丁区分 

分 类 号：TS262.5[轻工技术与工程—发酵工程] TS210.1[轻工技术与工程—食品科学与工程]