纤维素酶果胶酶辅助提取西瓜番茄红素工艺优化  

Optimization of Extraction of Lycopene from Watermelon Assisted by Cellulase and Pectinase

作　　者：刘义庆[1,2] 王春燕[3] 胡思前[1] LIU Yi-qing;WANG Chun-yan;HU Si-qian(Chemical and Environmental Engineering Institute of Jianghan University,Wuhan 430056,China;College of Food Science&Technology,Huazhong Agricultural University,Wuhan 430070,China;Jinan Fruit Research Institute,All China Federation of Supply&Marketing Co-operatives,Jinan 250014,China)

机构地区：[1]江汉大学化学与环境工程学院,湖北武汉430056 [2]华中农业大学食品科学技术学院,湖北武汉430070 [3]中华全国供销合作总社济南果品研究院,山东济南250014

出　　处：《中国果菜》2020年第8期13-17,51,共6页China Fruit & Vegetable

基　　金：湖北省科技创业服务能力建设专项基金(2018AKA172);武汉市高新技术产业发展计划现代农业专项基金资助(201220837304)。

摘　　要：本试验以质量比为4:1的纤维素酶、果胶酶组成的复合酶辅助提取西瓜番茄红素,分析了纤维素酶、果胶酶的最佳提取条件,分析了纤维素酶、果胶酶的最佳作用条件,采用正交试验优化了纤维素酶、果胶酶辅助提取西瓜番茄红素的工艺。结果得出,提取西瓜番茄红素最佳工艺为添加复合酶(维素酶:果胶酶=4:1)至西瓜糊(0.60%,w/w),调节体系pH值为4.5,55℃下酶解2.0 h;采用乙酸乙酯-丙酮混合溶剂(7:1,V/V)提取,提取率91%,提取效果较好。In order to optimize the process of extracting lycopene from watermelon,the optimum conditions of cellulase and pectinase were tested,and the effects of mixed enzyme on extraction efficiency were studied by orthogonal experiment method.The results showed that the lycopene was extracted effectively under following conditions:complexenzyme(cellulose and pectinase)mass ratio of 4:1,enzyme concentration of 0.60%,pH value of 4.5,and enzymatic hydrolysis for 2 h at 55℃,the extraction solvent of ethyl acetate and acetone with the volume ratio of 7:1.

关 键 词：番茄红素 酶解 西瓜 纤维素酶 果胶酶 

分 类 号：TS201.1[轻工技术与工程—食品科学]