具有抑菌特性的乳杆菌对食源性病原菌生物被膜形成的影响  

The Effect of Antibacteria Activity Lactobacillus on Biofilm Formation of Food-borne Pathogens

作　　者：张钰皎 陈忠军[1] 满都拉[1] 孙子羽 苏杰[2] 金晶晶 胡炜东[2] ZHANG Yu-jiao;CHEN Zhong-jun;Mandlaa;SUN Zi-yu;SU Jie;JIN Jing-jing;HU Wei-dong(College of Food Science and Engineering,Inner Mongolia Agricultural University,Huhhot 010018,Inner Mongolia,China;Vocational and Technical College,Inner Mongolia Agricultural University,Baotou 014109,Inner Mongolia,China)

机构地区：[1]内蒙古农业大学食品科学与工程学院,内蒙古呼和浩特010018 [2]内蒙古农业大学职业技术学院,内蒙古包头014109

出　　处：《食品研究与开发》2020年第22期168-174,共7页Food Research and Development

基　　金：内蒙古自治区高等学校科学研究项目(NJZZ17053)。

摘　　要：以金黄色葡萄球菌、大肠杆菌和沙门氏菌为指示菌,研究分离自内蒙古传统发酵食品中的5株乳杆菌对病原菌及其生物被膜的抑制作用。结果表明,5株乳杆菌对单一菌和混合菌生物被膜及AI-2分子相对活性均有抑制作用;ALAC-1和ALAC-5代谢产物抑菌效果较好,尤其对金黄色葡萄球菌抑制作用最好,ALAC-5抑菌率高达78.95%,ALAC-1可达69.47%;混合菌生物被膜对5株乳杆菌代谢产物的敏感性较弱,乳杆菌对其生物被膜抑制作用明显低于单一菌生物被膜。In the research,using Staphylococcus aureus,Escherichia and coli-Salmonella as the object,and the inhibitory effects of five Lactobacillus strains isolated from traditional fermented foods in Inner Mongolia on pathogens and their biofilms were studied.The results showed that 5 Lactobacillus strains inhibited the relative activities of single and mixed bacterial biofilms and AI-2 molecules;ALAC-1 and ALAC-5 metabolites had better antibacterial effects,especially against Staphylococcus aureus.Preferably,ALAC-5 had a bacteriostasis rate of 78.95%and ALAC-1 could reach 69.47%.The mixed biofilm was sensitive to 5 Lactobacillus metabolites,and the inhibition effect of Lactobacillus on its biofilm was significantly lower than that of single.

关 键 词：乳杆菌 生物被膜 抑菌特性 群体感应 混合菌生物被膜 

分 类 号：TS201.3[轻工技术与工程—食品科学]