啤酒花中不同比例β-酸同系物的抗氧化及抑菌活性  

Antioxidant and Antibacterial Activities of β-Acid Homologue Mixtures with Different Ratios from Hops

作　　者：杨静文 刘泽畅 陈培 杜伟栋 范效兰 石铭 刘玉梅[1] YANG Jingwen;LIU Zechang;CHEN Pei;DU Weidong;FAN Xiaolan;SHI Ming;LIU Yumei(School of Chemical Engineering and Technology,Xinjiang University,Ürümqi 830046,China)

机构地区：[1]新疆大学化工学院,新疆乌鲁木齐830046

出　　处：《食品科学》2020年第23期83-90,共8页Food Science

基　　金：国家大学生创新训练计划项目(201810755046);国家自然科学基金地区科学基金项目(31660490)。

摘　　要：啤酒花软树脂中β-酸主要由合蛇麻酮、正蛇麻酮和加蛇麻酮3种同系物组成,啤酒花的品种不同,上述同系物会存在一定的比例差异。为探究啤酒花中不同比例β-酸同系物的抗氧化及抑菌活性,本实验在分离得到β-酸粗提物的基础上,采用结晶法获取了5种不同比例β-酸同系物样品,并通过测定黄嘌呤氧化酶活性抑制能力、1,1-二苯基-2-三硝基苯肼自由基和羟自由基清除能力、总抗氧化活性以及抑菌圈直径,对上述样品抗氧化活性和抑菌活性的差异进行比较研究。结果表明:不同比例β-酸同系物样品在抗氧化活性方面存在较大差异,其中合蛇麻酮与正蛇麻酮+加蛇麻酮的质量比为1∶4时,样品的抗氧化活性最强,说明正蛇麻酮+加蛇麻酮的抗氧化活性强于合蛇麻酮。在较高质量浓度(≥40.00 mg/L)时,高比例合蛇麻酮的β-酸样品抑菌活性均较强。除合蛇麻酮与正蛇麻酮+加蛇麻酮的质量比为1∶4的样品外,质量浓度为160.00 mg/L的样品与同质量浓度下阳性对照阿莫西林的抑菌效果相当,甚至更高;高比例正蛇麻酮+加蛇麻酮的β-酸样品对金黄色葡萄球菌的最小抑菌浓度最低,仅为5.00 mg/L,此外,尽管该样品对大肠杆菌的最小抑菌浓度与其余样品均为10.00 mg/L,但其抑菌圈直径更大。Hops soft resin contains three homologues ofβ-acids:co-,n-and ad-lupulone,and the ratio among these homologues varies depending on hops varieties.In this experiment,fiveβ-acid homologue mixtures were obtained from the crudeβ-acid from hops by crystallization,and their antioxidant activity in vitro were investigated comparatively by measuring the inhibitory activity against xanthine oxidase,1,1-diphenyl-2-picrylhydrazyl(DPPH)and OH radical scavenging activity,total antioxidant activity and inhibition zone diameter.The results showed that the antioxidant activity of theseβ-acid homologue mixtures were significantly different.Among them,the 1:4 mixture of co-lupulone with n-lupulone+ad-lupulone possessed the strongest antioxidant activity,indicating that the antioxidant activity of n-lupulone+ad-lupulone was stronger than that of co-lupulone.At high concentrations(≥40.00 mg/L),mixtures with higher proportion of co-lupulone had stronger antibacterial activity.The antibacterial activity of all samples except the 1:4 mixture of co-lupulone with n-lupulone+ad-lupulone at a concentration of 160.00 mg/L was similar to or even stronger than that of amoxicillin.The sample with the highest proportion of n+ad-lupulone showed the lowest minimum inhibitory concentration(5.00 mg/L)against Staphylococcus aureus.In addition,although the minimum inhibitory concentrations of all samples against Escherichia coli were equally 10.00 mg/L,this one exhibited a bigger diameter of inhibition zone.

关 键 词：啤酒花 软树脂 β-酸同系物 抗氧化活性 抑菌活性 

分 类 号：TS202.3[轻工技术与工程—食品科学]