NanoSe-LMH-CS-SA复合载药微球的制备与缓释性能研究  

Preparation and sustained-release profile of NanoSe-LMH-CS-SA drug-loading microsphere

作　　者：吴永军[1] 吉民 刘燕[1] 刘红梅[1] WU Yong-jun;JI Min;LIU Yan;LIU Hong-mei(Food Engineering Department,Maanshan Teacher’s College,Maanshan 243041,China;Maanshan BBCA Pharmaceutical Co.,Ltd.,Maanshan 243000,China)

机构地区：[1]马鞍山师范高等专科学校食品工程系,马鞍山243041 [2]马鞍山丰原制药有限公司,马鞍山243000

出　　处：《天然产物研究与开发》2020年第12期2122-2131,共10页Natural Product Research and Development

基　　金：安徽省高校优秀青年人才支持计划重点项目(gxyqZD2016580);安徽省高校省级自然科学重点研究项目(KJ2014A246)。

摘　　要：以壳聚糖(CS)、海藻酸钠(SA)为壁材,纳米硒(NanoSe)为芯材,L-盐酸赖氨酸(LMH)作为复合营养物,使用复凝法制备NanoSe-LMH-CS-SA复合载药微球。分析了载药微球的SEM形貌、粒度分布、红外光谱、热稳定性和元素组成,在单因素释放实验的基础上使用BBD响应面优化制备条件为:壁材比nCS/nSA=0.82、芯壁比nNanoSe/nCS+nSA=1.22、交联剂CaCl2用量0.86 g(8.6 mg/mL)、复凝pH=4.38,对应包埋率为84.65%。体外模式消化实验证实,5 h内此种优化载药微球在模拟胃液和模拟小肠液中的累积缓释率达到73.08%和57.95%;7 h内达到78.57%和65.80%。NanoSe-LMH-CS-SA drug-loading microsphere was prepared through the complex coacervation method using chitosan and sodium alginate as the wall materials,NanoSe as the core materials and LMH as the composite nutrient.These properties such as SEM morphology,particle size distribution,infrared spectroscopy,thermal stability and element composition were analyzed to describe the microspheres structure.Based on the single factor in vitro release experiments,the preparation technology optimized by Box-Benhnken response surface design was:the ratio of wall material to wall material of 0.82(nCS/nSA=0.82),the ratio of core material to wall material of 1.22(nNanoSe/nCS+nSA=1.22),adding 0.86 g CaCl2 as crosslinking agent,the complex coacervation pH ranging was 4.28,and the embedding rate of drug-loading microsphere was 84.65%.Simulated digestion experiments showed that the cumulative rate of slow release of optimized drug-loading microsphere were 73.08%(simulated gastric juice)and 57.95%(simulated small intestinal juice)in 5 h,78.57%(simulated gastric juice)and 65.80%(simulated small intestinal juice)in 7 h.

关 键 词：纳米硒 赖氨酸 壳聚糖 海藻酸钠 缓释 响应面设计 

分 类 号：TS201.2[轻工技术与工程—食品科学] O613.52[轻工技术与工程—食品科学与工程]