黑茶超高压和热水提取物的体外降血糖活性研究  

In Vitro Assessment of Hypoglycaemic Activity of Dark Tea Extracts by Ultra-High Pressure Technique and Hot Water Bath

作　　者：苏可盈 李倩 赵世民 王凯[2] 张学武[2] SU Keying;LI Qian;ZHAO Shimin;WANG Kai;ZHANG Xuewu(Guangzhou College of Technology and Business,Guangzhou 510850,China;South China University of Technology,Guangzhou 510642,China)

机构地区：[1]广州工商学院,广东广州510850 [2]华南理工大学,广东广州510642

出　　处：《食品科技》2020年第11期237-242,共6页Food Science and Technology

基　　金：广州市产学研协同创新重大专项(201704020024)。

摘　　要：将超高压技术和热水提取法用于黑毛尖(HMJ)和千两茶(QL)活性物质的提取,并测定了各种黑茶提取物对α-葡萄糖苷酶和二肽基肽酶(DPP4)的抑制作用。结果表明,超高压提取法得率为13.57%~14.22%,高于热水提取法的得率(10.45%~12.45%)。对α-葡萄糖苷酶的抑制作用最强的是千两茶的热水提取物(IC50=4.91×10^—5 mg/mL),2 mg/mL千两茶热水提取物的抑制率达98.51%,相同浓度的千两茶超高压提取物的抑制率仅达63.57%。对DPP4抑制作用最强的是黑毛尖的热水提取物(IC50=2.9×10^—1 mg/mL),2 mg/mL黑毛尖热水提取物的抑制率达71.14%,相同浓度的千两茶热水提取物的抑制率仅达59.26%。可见,黑茶提取物有显著的降血糖作用,可用于降血糖保健食品或药品的开发,但黑茶的品种和提取方法对其降血糖活性影响较大。To study influence of extraction methods on hypoglycemic activity of different varieties of dark tea,ultrahigh pressure(UHP)technique and hot water bath were applied to two kinds of dark tea extraction,Heimaojian(HMJ)and Qianliang(QL).The inhibiton activity of dark tea extracts onα-glucosidase and DPP4 were determined.Consequently,UHP technique gained extraction rate of 13.57%~14.22%,higher than hot water bath(10.45%~12.45%).Hot water bath QL extract showed the greatest potency onα-glucosidase inhibiton with an IC50 value of 4.91×10^—5 mg/mL,and 98.51%inhibition rate when present at a concentration of 2 mg/mL,while the inhibition rate for UHP QL extract is 63.57%.Moreover,hot water bath HMJ extract exhibited highest DPP4 inhibition activities with the IC50 values of 2.9×10^—1 mg/mL,whose inhibitory rate was 71.14%at the concentration of 2 mg/mL,while the inhibition rate for hot water bath QL extract is 59.26%.In conclusion,the extraction methods and dark tea varieties have great effect on hypoglycemic activity,and all dark tea extracts displayed inhibition on two emzymes,so they could be good raw material for hypoglycemic agents.

关 键 词：黑茶 降血糖 超高压 Α-葡萄糖苷酶 二肽基肽酶(DPP4) 

分 类 号：R284.2[医药卫生—中药学]