酿酒酵母高级醇代谢研究进展  

Higher alcohols metabolism by Saccharomyces cerevisiae:a mini review

作　　者：孙中贯[1,2,3,4] 刘琳 王亚平 王雪山[3,4] 肖冬光 Zhongguan Sun;Lin Liu;Yaping Wang;Xueshan Wang;Dongguang Xiao(College of Biotechnology,Tianjin University of Science and Technology,Tianjin 300457,China;Key Laboratory of Industrial Fermentation Microbiology,Ministry of Education,Tianjin University of Science and Technology,Tianjin 300457,China;College of Food Science and Pharmaceutical Engineering,Zaozhuang University,Zaozhuang 277160,Shandong,China;Key Laboratory of Wuliangye-flavor Liquor Solid-state Fermentation,China National Light Industry,Yibin 644000,Sichuan,China;College of Life Science,Zaozhuang University,Zaozhuang 277160,Shandong,China)

机构地区：[1]天津科技大学生物工程学院,天津300457 [2]天津科技大学工业发酵微生物教育部重点实验室,天津300457 [3]枣庄学院食品科学与制药工程学院,山东枣庄277160 [4]中国轻工业浓香型白酒固态发酵重点实验室,四川宜宾644000 [5]枣庄学院生命科学学院,山东枣庄277160

出　　处：《生物工程学报》2021年第2期429-447,共19页Chinese Journal of Biotechnology

基　　金：国家自然科学基金(No.31771969);中国轻工业浓香型白酒固态发酵重点实验室开放基金(No.2018JJ018)资助。

摘　　要：高级醇是酿酒酵母在饮料酒酿造过程中产生的主要代谢副产物之一。饮料酒中高级醇含量过高,易导致饮用后产生头痛、口渴等症状,是醉酒较慢、醉酒后较难醒酒的主要原因。文中系统综述了饮料酒中主要高级醇的风味特征、代谢途径及诱变育种技术在酿酒酵母高级醇代谢调控中的应用,特别阐述了代谢工程技术在氨基转移酶编码基因、α-酮酸代谢基因、乙酸酯代谢基因与碳氮代谢基因改造中的应用,并对未来实现高级醇代谢途径精细化调控的发展方向进行了展望。文中总结对于酿酒酵母高级醇代谢调控系统的建立具有重要的理论意义,对于适量产生高级醇的酿酒酵母工业菌株的选育具有重要的实际指导意义。Higher alcohols are one of the main by-products of Saccharomyces cerevisiae in brewing.High concentration of higher alcohols in alcoholic beverages easily causes headache,thirst and other symptoms after drinking.It is also the main reason for chronic drunkenness and difficulty in sobering up after intoxication.The main objective of this review is to present an overview of the flavor characteristics and metabolic pathways of higher alcohols as well as the application of mutagenesis breeding techniques in the regulation of higher alcohol metabolism in S.cerevisiae.In particular,we review the application of metabolic engineering technology in genetic modification of amino transferase,α-keto acid metabolism,acetate metabolism and carbon-nitrogen metabolism.Moreover,key challenges and future perspectives of realizing optimization of higher alcohols metabolism are discussed.This review is intended to provide a comprehensive understanding of metabolic regulation system of higher alcohols in S.cerevisiae and to provide insights into the rational development of the excellent industrial S.cerevisiae strains producing higher alcohols.

关 键 词：酿酒酵母 高级醇 饮料酒 代谢 精细化调控 

分 类 号：TQ923[轻工技术与工程—发酵工程]