山西老陈醋源植物乳杆菌C181对小鼠酒精肝损伤的保护作用  

Protective Effects of Lactobacillus plantarum C181 Isolated from Shanxi Aged Vinegar on Ethanol-induced Liver Injury in Mice

作　　者：许女[1] 孙建平 陈旭峰[1] 王佳丽[1] 贾瑞娟 张浩[1] 王如福[1] Xu Nü;Sun Jianping;Chen Xufeng;Wang Jiali;Jia Ruijuan;Zhang Hao;Wang Rufu(Food Science and Engineering College,Shanxi Agricultural University,Taigu 030801,Shanxi)

机构地区：[1]山西农业大学食品科学与工程学院,山西太谷030801

出　　处：《中国食品学报》2021年第2期121-127,共7页Journal of Chinese Institute Of Food Science and Technology

基　　金：山西省重点研发计划项目(201903D221037)。

摘　　要：本文研究了山西老陈醋源植物乳杆菌C181对小鼠酒精肝损伤的保护作用。研究结果显示,灌胃植物乳杆菌C181可显著降低慢性酒精诱导小鼠肝脏中甘油三酯和游离脂肪酸的含量,血清中谷草转氨酶(AST)和谷丙转氨酶(ALT)活性也降低了。植物乳杆菌C181通过调节肝脏中Nrf-2和NF-κB基因的表达水平,调节肝脏的氧化应激超氧化物歧化酶(SOD)、谷胱甘肽(GSH)活性和丙二醛(MDA)含量以及炎症因子(TNF-α、IL-6、IFN-γ和IL-10)的分泌,改善酒精肝损伤。本文还研究了植物乳杆菌C181对酒精肝损伤小鼠肠道菌群及肠道紧密连接蛋白相关基因ZO-1、Claudin和Occludin表达的影响,结果表明植物乳杆菌C181通过维持肠道菌群平衡保护肠上皮细胞的屏障功能,达到预防酒精性肝病的目的。The protective effects of L.plantarum C181 against chronic alcohol-induced liver injury in mice were investigated.Oral administration of L.plantarum C181 significantly decreased the levels of liver triglyceride and free fatty acid,also,serum alanine transaminase(ALT)and aspartate aminotransferase(AST)were decreased in chronic alcohol-challenged mice.L.plantarum C181 was also found to suppress the content of malondialdehyde(MDA)and enhance the activity of superoxide dismutase(SOD)and glutathion reductase(GSH)in liver,which are well-correlated with the upregulation of hepatic nuclear factor erythroid 2-related factor(Nrf-2)and downregulation of(NF-κB)mRNA.Moreover,L.plantarum C181 significantly decreased levels of TNF-α、IL-6 and IFN-γand increased levels of IL-10 in liver.Additionally,Effect of L.plantarum C181 on intestinal floras and ZO-1,Occludin and Claudin mRNA were studied.These results furtherly demonstrate that L.plantarum C181 could efficiently protects against alcoholic chronic liver injury through recovery of intestinal flora balance and intestinal barrier function.

关 键 词：植物乳杆菌 酒精肝损伤 

分 类 号：TS264.22[轻工技术与工程—发酵工程]