青稞膳食纤维和多酚对肠道微生物的协同调节作用  

Synergetic regulation of gut microbiota by dietary fiber and phenolic compounds in hulless barley

作　　者：鲁朝凤 黄佳琦 黄勇桦 杨士花[4] 陈壁 杨明静 李永强[1] LU Chaofeng;HUANG Jiaqi;HUANG Yonghua;YANG Shihua;CHEN Bi;YANG Mingjing;LI Yongqiang(College of Food Science and Technology,Yunnan Agricultural University,Kunming 650201,China;Hangzhou Hanku Medical Laboratory Limited Company,Hangzhou 310000,China;West Yunnan University of Applied Sciences College of Tea(Pu′er),Pu′er 665099,China;College of Foreign Languages,Yunnan Agricultural University,Kunming 650201,China)

机构地区：[1]云南农业大学食品科学技术学院,云南昆明650201 [2]杭州汉库医学检验所有限公司,浙江杭州310000 [3]滇西应用技术大学普洱茶学院,云南普洱665099 [4]云南农业大学外语学院,云南昆明650201

出　　处：《食品与发酵工业》2021年第8期6-13,共8页Food and Fermentation Industries

基　　金：国家自然科学基金(31560428,31360378)。

摘　　要：以青稞面粉(hulless barley flour,HBF)、不可溶膳食纤维-多酚复合物(insoluble dietary fiber-phenolic compounds,IDF-PC)和可溶性膳食纤维-多酚复合物(soluble dietary fiber-phenolic compounds,SDF-PC)为材料,以阿魏酸(ferulic acid,FA)为对照,采用酶解法制备IDF-PC和SDF-PC,Folin-Ciocalteu法测定多酚含量,利用体外结肠发酵,结合16S rRNA高通量测序,根据微生物的丰度和多样性分析青稞膳食纤维和多酚对肠道微生物协同调节作用,并利用高效液相色谱法进行短链脂肪酸分析。结果表明,HBF、IDF-PC和SDF-PC中含有丰富的膳食纤维和多酚。经体外结肠发酵后,3种样品能够提高肠道微生物的丰度和多样性,明显促进乳杆菌属(Lactobacillus)、艾克曼菌属(Akkermansia)、瘤胃菌科UCG-005(Ruminococcaceae-UCG-005)和瘤胃菌科NK4A214-(Ruminococcaceae-NK4A214-group)的生长,调节作用优于FA。HBF、IDF-PC和SDF-PC经体外结肠发酵后,能够产生较多的短链脂肪酸,乙酸含量最高,分别为(0.29±0.21)、(0.15±0.05)和(0.14±0.10)mg/mL,说明青稞膳食纤维和多酚对肠道微生物有较好的协同调节作用,可为青稞促进机体健康和预防疾病提供科学依据。In this study,hulless barley flour(HBF),insoluble dietary fiber-phenolic compounds(IDF-PC)and soluble dietary fiber-phenolic compounds(SDF-PC)were used as experimental materials to analyze the synergetic regulation of gut microbiota by dietary fiber and polyphenol,according to the abundance and diversity of microbes by in vitro colonic fermentation and 16S rRNA high-throughput sequencing.Ferulic acid(FA)was taken as the control.IDF-PC and SDF-PC were extracted and isolated by enzymatic hydrolytic methods.The total phenolic content was determined using Folin-Ciocalteu assay.Moreover,short-chain fatty acids were analyzed by high performance liquid chromatography.The results showed that HBF,IDF-PC and SDF-PC were rich in dietary fibers and phenolic compounds.After in vitro colonic fermentation,these three samples could improve the abundance and diversity of gut microbiota and promote the growth of bacterial groups,including Lactobacillus,Akkermansia,Ruminococcaceae-UCG-005,Ruminococcaceae-NK4A214-group,compared with FA.Fermentation of HBF,IDF-PC and SDF-PC resulted in an increase in SCFAs production and the peak contents in acetic acid,which were(0.29±0.21),(0.15±0.05)and(0.14±0.10)mg/mL,respectively.This study suggests that hulless barley dietary fibers and phenolic compounds have better synergistic regulation on gut microbiota,which can provide scientific basis for hulless barley health promotion and disease prevention.

关 键 词：青稞 膳食纤维 多酚 体外结肠发酵 肠道微生物 协同调节 

分 类 号：TS201.4[轻工技术与工程—食品科学]