海洋来源产低温几丁质酶菌株的诱变选育及酶学性质研究  

Mutation Breeding and Enzymatic Properties of Cold-adapted Chitinolytic Strain Photobacterium sp.from Marine

作　　者：陈立功 吴家葳 张金平 张庆芳[1] 迟乃玉[1] 王晓辉[1] 王梦雨 CHEN Ligong;WU Jiawei;ZHANG Jinping;ZHANG Qingfang;CHI Naiyu;WANG Xiaohui;WANG Mengyu(Dalian Key Laboratory of Synthetic Biology,Liaoning Marine Microbial Engineering Technology Research Center,School of Life Science and Biotechnology,Dalian University,Dalian 116600,China)

机构地区：[1]大连大学生命科学与技术学院,辽宁省海洋微生物工程技术研究中心,大连市合成生物学重点实验室,辽宁大连116600

出　　处：《食品工业科技》2021年第10期105-111,共7页Science and Technology of Food Industry

基　　金：国家自然科学基金青年科学基金(31500039);辽宁省博士启动基金指导计划项目(20170520167);大连市青年科技之星项目(2017RQ155);大连大学博士专项基金(2017QL020);辽宁省自然科学基金(20180550728);省自然科学基金指导计划(20180550728);高层次人才创新支持计划(2018RQ44)。

摘　　要：为研究菌株Photobacterium sp. LG-1理化性质,提高其产酶活性。利用BIOLOG对其进行生理生化实验,通过紫外诱变、化学诱变及复合诱变进行育种,并对其进行酶学性质及抑菌性研究。结果表明,该菌株复合诱变后酶活达2.690 U/mL,酶活提高率为139.78%;最适反应温度为35℃,0℃仍具有催化活性;最适反应pH为8.0,在偏碱性环境中有较好的稳定性;Ca^(2+)、K^(+)、Mg^(2+)、Cu^(2+)、Na^(+)对几丁质酶活性有促进作用。LG-1对根霉、黑曲霉、毛霉及玉米腐烂病有抑制活性。To study the physicochemical properties of the strain Photobacterium sp. LG-1 and improve its enzyme production activity, BIOLOG was used to carry out physiological and biochemical experiments. Parallel breeding through ultraviolet mutagenesis, chemical mutagenesis and compound mutagenesis. Research on its enzymatic properties and antibacterial properties. The enzyme activity of this strain reached 2.690 U/mL after compound mutagenesis, and the increasing rate of enzyme activity was 139.78%. The optimum temperature was 35℃, and the chitinase still showed catalytic activity at 0 ℃. The optimum pH was 8.0, and it had stability in an alkaline environment. Ca2+, K+, Mg2+, Cu2+ and Na+ could promote chitinase activity. LG-1 had inhibitory activity against Rhizopus, Aspergillus niger, Mucor and rot of Corn.

关 键 词：发光杆菌 低温几丁质酶 生理生化 酶学性质 抑菌活性 

分 类 号：TS254.1[轻工技术与工程—水产品加工及贮藏工程]