响应面试验优化草鱼鳞胶原蛋白钙结合肽的制备  

Optimization of Enzymatic Hydrolysis of Calcium Binding Peptides of Collagen from Grass Fish Scales

作　　者：许青[1] 徐昕[1] 公丽艳 张叶帆 杨平[1] XU Qing;XU Xin;GONG Liyan;ZHANG Yefan;YANG Ping(Shenyang Normal University,Shenyang 110034)

机构地区：[1]沈阳师范大学,沈阳110034

出　　处：《食品工业》2021年第3期1-4,共4页The Food Industry

基　　金：辽宁省科技厅项目(2020JH2/10200041);沈阳师范大学创新训练项目(x202010166088)。

摘　　要：为实现草鱼鳞蛋白的高值化利用,经蛋白酶筛选,在单因素试验基础上,以钙结合率为主要指标,水解度为辅助指标,研究双酶质量比、酶解温度、酶解时间对胶原蛋白酶解物钙结合率和水解度的影响。结果表明,复合蛋白酶与风味蛋白酶是草鱼鳞胶原蛋白酶解的最适用酶,以酶质量比、底物质量浓度及水解温度为自变量的数学模型,得到酶质量比6︰4、底物质量浓度18.75 g/L、温度51.5℃条件下酶解肽的结合钙量为33.43%。In order to realize the high value utilization of collagen of grass fish scale, after selection of an appropriate enzyme, on the basis of single factor experiment, the effects of double enzyme mass ratio, substrate concentration and hydrolysis temperature on calcium binding mass and the degree of hydrolysis were investigated by using response surface methodology. The results showed that complex proteases and flavor proteases were the best enzyme for the hydrolysis of calcium binding peptides of collagen. The optimal condition that calcium binding activity was determined as follows: double enzyme mass ratio 6︰4, substrate concentration 18.75 g/L, hydrolysis temperature 51.5 ℃. Under this condition, the bound calcium content by the hydrolysate was up to 33.43%.

关 键 词：酶解 鱼鳞 钙结合肽 

分 类 号：TS254.9[轻工技术与工程—水产品加工及贮藏工程]