牡蛎肽对TM3细胞性功能的影响  

Effects of Oyster Peptide on Cellular Function of TM3

作　　者：方磊 张瑞雪 陈亮[1] 张海欣[1] 贾福怀 晏永球 谷瑞增[1] 魏颖[1] Fang Lei;Zhang Ruixue;Chen Liang;Zhang Haixin;Jia Fuhuai;Yan Yongqiu;Gu Ruizeng;Wei Ying(China National Research Institute of Food and Fermentation,Beijing Engineering Research Center of Protein&Functional Peptides,Beijing 100015;Ningbo Yufangtang Biological Technology Co.Ltd.,Ningbo 315012,Zhejiang)

机构地区：[1]中国食品发酵工业研究院北京市蛋白功能肽工程技术研究中心,北京100015 [2]宁波御坊堂生物科技有限公司,浙江宁波315012

出　　处：《中国食品学报》2021年第5期140-147,共8页Journal of Chinese Institute Of Food Science and Technology

基　　金：“十三五”国家重点研发计划项目(2016YFD0400604)。

摘　　要：分析牡蛎全粉、牡蛎肽的氨基酸组成和分子质量分布,研究不同质量浓度牡蛎全粉及牡蛎肽对小鼠睾丸间质细胞(TM3细胞)合成分泌睾酮、二氢睾酮、环磷酸鸟苷(cGMP)等影响,为牡蛎肽改善性功能提供依据。采用四甲基偶氮唑蓝(MTT)比色法测定牡蛎全粉和牡蛎肽对TM3细胞存活率的影响,酶联免疫吸附法检测睾酮、二氢睾酮、cGMP的含量,硝酸盐还原酶法检测一氧化氮(NO)的含量,氮蓝四唑(NBT)显色法检测细胞内SOD的酶活力。结果表明:牡蛎全粉和牡蛎肽的氨基酸组成丰富,牡蛎肽含有更多人体必需氨基酸和精氨酸,牡蛎肽中分子质量小于1000 u的组分高达92.1264%。与牡蛎全粉相比,牡蛎肽能够更显著地促进TM3细胞分泌睾酮、二氢睾酮、cGMP,显著增强TM3细胞内NO的合成,提高TM3细胞的SOD酶活力,表明牡蛎肽具有促进性功能的作用,其机制可能与牡蛎肽改善NO-cGMP通路有关。The amino acid composition and molecular weight distribution of oyster whole powder and oyster peptide were analyzed.The effects of different concentrations of oyster powder and oyster peptide on the synthesis and secretion of testosterone,dihydrotestosterone and cyclic guanosine monophosphate(cGMP)in mouse leydig cells(TM3 cells)were studied to provide a basis for improving the sexual function of oyster peptides.The effects of oyster whole powder and oyster peptide on the survival rate of TM3 cells were determined by MTT colorimetric assay.The contents of testosterone,dihydrotestosterone and cGMP were detected by enzyme-linked immunosorbent assay.Nitrate reductase method was used to determine the content of nitric oxide(NO)and the activity of SOD in the cells was detected by nitroblue tetrazolium(NBT)chromogenic assay.The results showed that the oyster whole powder and oyster peptide had rich amino acid composition,the oyster peptide contained more essential amino acids and arginine,and the oyster peptide had a molecular weight of less than 1000u as high as 92.1264%.Compared with oyster whole powder,oyster peptide could significantly promote the secretion of testosterone,dihydrotestosterone and cGMP in TM3 cells,significantly enhance the synthesis of NO in TM3 cells,and increase the SOD activity of TM3 cells,indicating that oyster peptides had promoting functions.The role of its mechanism may be related to the improvement of the NO-cGMP pathway by oyster peptides.

关 键 词：牡蛎肽 性功能 睾酮 CGMP 

分 类 号：TS254.1[轻工技术与工程—水产品加工及贮藏工程]