速溶藏茶制备工艺优化、功能性成分及抗氧化性分析  

Optimization of Preparation Technology of Instant Tibetan Tea and Analysis of Its Functional Components and Antioxidant Activity

作　　者：张恒[1] 郑俏然[2] 何靖柳[1] 韦婷[1] 刘翔[1] 章斌[1] ZHANG Heng;ZHENG Qiaoran;HE Jingliu;WEI Ting;LIU Xiang;ZHANG Bin(School of Pharmacy and Medical Laboratory Science,Ya'an Polytechnic College,Ya'an 625000,China;Life Science and Technology Institute,Yangtze Normal University,Chongqing 408100,China)

机构地区：[1]雅安职业技术学院药学与检验学院,四川雅安625000 [2]长江师范学院生命科学与技术学院,重庆408100

出　　处：《食品工业科技》2021年第11期147-155,共9页Science and Technology of Food Industry

基　　金：四川省教育厅自然科学项目(18ZB0655);雅安市应用技术研究与开发资金项目(2017yyjskf02)。

摘　　要：为制备速溶藏茶,本研究以速溶藏茶得率、藏茶多糖提取量和藏茶茶多酚提取量为指标,通过单因素实验和响应面试验优化了速溶藏茶浸提工艺,并进一步研究了冷冻干燥和喷雾干燥对速溶藏茶中功能性成分和体外抗氧化活性的影响。结果表明,藏茶最佳浸提条件为料液比1:53 (g/mL),温度98.5℃,浸提时间1.5 h,浸提次数3次,此时速溶藏茶得率为29.72%±0.13%,藏茶多糖提取量为6.06±0.03(g/100g),藏茶茶多酚提取量为6.27±0.05(g/100g)利用UHPLC-QqQ-MS/MS检测出冷冻干燥和喷雾干燥的速溶藏茶中均有10种功能性成分,EGCG、GA、GCG、ECG为速溶藏茶主要功能性成分,且冷冻干燥的速溶藏茶中EGC、EGCG和槲皮素含量更高(P<0.05)。冷冻干燥速溶藏茶对DPPH自由基和ABTS自由基清除能力的IC_(50)分别为0.247、0.417 mg/mL;喷雾干燥速溶藏茶对DPPH自由基和ABTS自由基清除能力的IC_(50)分别为0.339、0.443 mg/mL,可见,速溶藏茶具有较好的抗氧化活性。To prepare instant Tibetan tea,the extraction technology was optimized by single factor experiment and BoxBehnken response surface design,with the yield of instant Tibetan tea,the extraction of polysaccharide and the extraction of polyphenols as the evaluation index.The results showed that the optimal extraction conditions were as follows:solid-liquid ratio 1:53(g/mL),extraction temperature 98.5℃,extraction time 1.5 h,and extraction 3 times.Under the optimal extraction conditions,the yield of instant Tibetan tea was 29.72%±0.13%,the extraction of polysaccharide was(6.06±0.03)g/100 g,and the extraction of Tibetan tea polyphenols was(6.27±0.05)g/100 g.Ten kinds of functional components in instant Tibetan tea were detected by UHPLC-QqQ-MS/MS,EGCG,GA,GCG and ECG were the main catechins.The contents of EGC,EGCG,quercetin in freeze-dried instant Tibetan tea were higher than those in spray-dried instant Tibetan tea(P<0.05).The IC50 of freeze-dried instant tea on scavenging ability of DPPH and ABTS free radicals were 0.247 and 0.417 mg/mL,and the IC50 of spray-dried instant tea on scavenging ability of DPPH and ABTS free radicals were 0.339 and 0.443 mg/mL.Therefore,the instant Tibetan tea powder has good antioxidant activity.

关 键 词：速溶藏茶 茶多酚 藏茶多糖 功能性成分 抗氧化性 干燥方式 

分 类 号：TS201.2[轻工技术与工程—食品科学]