高效液相色谱法测定速溶滇红茶制品中茶黄素含量  

Determination of theaflavins in instant Yunnan black tea products by high performance liquid chromatography

作　　者：刘雪娜 吴雪娇 刘顺航 卢念东 李丽维 LIU Xue-Na;WU Xue-Jiao;LIU Shun-Hang;LU Nian-Dong;LI Li-Wei(Tasly Academy,Tianjin 300410,China;Yunnan Tasly Deepuer Biological Tea Group Company Limited,Puer 665000,China)

机构地区：[1]天士力研究院,天津300410 [2]云南天士力帝泊洱生物茶集团有限公司,普洱665000

出　　处：《食品安全质量检测学报》2021年第9期3807-3812,共6页Journal of Food Safety and Quality

摘　　要：目的建立高效液相色谱法测定速溶滇红茶制品中茶黄素含量的分析方法。方法样品提取后,采用Agilent ZORBAX SB-C18色谱柱分离,以0.1%甲酸乙腈-0.1%甲酸水溶液为流动相进行梯度洗脱,流速为1 mL/min,柱温30℃,检测波长为280 nm,外标法定量。结果4种茶黄素单体在各自浓度范围内线性关系良好,相关系数均大于0.9995,检出限为1.1~1.6μg/g,定量限为3.7~5.5μg/g,加标回收率为95.08%~104.50%,相对标准偏差均小于2.01%。结论本检测方法具有较好的重复性、精密度及稳定性,适用于检测速溶滇红茶制品中茶黄素的含量。Objective To establish a method for the determination of theaflavins in instant Yunnan black tea products by high performance liquid chromatography.Methods After extraction,the samples were separated by Agilent ZORBAX SB-C18 column using 0.1%formic acid acetonitrile-0.1%formic acid aqueous solution as the mobile phase for gradient elution.The flow rate was 1 mL/min,the column temperature was 30℃,and the detection wavelength was 280 nm.The samples were quantified by external standard method.Results The 4 theaflavins had good linear relationships within their respective concentration ranges,the correlation coefficients were all greater than 0.9995,the limits of detection were 1.1-1.6μg/g,the limits of quantification were 3.7-5.5μg/g,the standard recoveries were 95.08%-104.50%,and the relative standard deviations were less than 2.01%.Conclusion This method has good repeatability,precision and stability,and is suitable for the determination of theaflavins in Yunnan black instant tea products.

关 键 词：高效液相色谱法 滇红茶 速溶茶 茶黄素 

分 类 号：TS272.52[农业科学—茶叶生产加工] O657.72[轻工技术与工程—农产品加工及贮藏工程]