乳酸菌抗氧化特性及其katA基因分析  

Antioxidant Properties of Lactobacillus and Its katA Gene Analysis

作　　者：张开屏[1] 赵艳红 李权威 闫娜 吕金积 张海荣 田建军[2] 靳烨[2] Zhang Kaiping;Zhao Yanhong;Li Quanwei;Yan Na;LüJinji;Zhang Hairong;Tian Jianjun;Jin Ye(Department of Food Engineering,Inner Mongolia Business Vocational College,Hohhot 010070;College of Food Science and Engineering,Inner Mongolia Agricultural University,Hohhot 010018;Inner Mongolia Grassland Jingxin Food Co.,Ltd.,Bayannur 015000,Inner)

机构地区：[1]内蒙古商贸职业学院食品工程系,呼和浩特010070 [2]内蒙古农业大学食品科学与工程学院,呼和浩特010018 [3]内蒙古草原晶鑫食品有限公司,内蒙古巴彦淖尔015000

出　　处：《中国食品学报》2021年第7期37-43,共7页Journal of Chinese Institute Of Food Science and Technology

基　　金：国家自然科学基金地区项目(31960514);内蒙古自治区科技计划项目(2019GG239)。

摘　　要：为了分析乳杆菌的抗氧化特性及其与katA基因的关系,以自然发酵肉制品中筛选的6株乳杆菌为研究对象,通过对1,1-二苯基-2-苦基肼(DPPH)、羟(·OH)、超氧阴离子(O_(2)^(-)·)自由基清除率、H_(2)O_(2)耐受性以及katA基因的表达进行评价。结果表明:试验菌株清除·OH自由基的活性物质主要是菌体细胞及胞内物质,而清除DPPH和O_(2)^(-)·自由基主要是乳酸菌的代谢产物;TR1-1-3对3种自由基的清除能力都比较强,而菌株X31对·OH和O_(2)^(-)·的清除率最低,且与TR1-1-3差异显著(P<0.05);除菌株X31外,其它试验菌株在H_(2)O_(2)浓度0~1.0 mmol/L范围,对H_(2)O_(2)均有一定的耐受能力,且在12~48 h培养时间内,随着培养时间的延长,耐受性逐渐增强。对自由基清除率和H_(2)O_(2)耐受能力弱的菌株X31的katA基因的特异性PCR检测结果为阴性,而其它菌株均为阳性。本研究发现乳酸菌抗氧化能力具有菌株特异性,通过分析乳酸菌对O_(2)^(-)·、·OH、DPPH自由基的清除率以及对H_(2)O_(2)耐受性的表观数据,结合katA基因表达情况,可以作为乳酸菌抗氧化能力强弱判定的检测方法。研究结果可为优质菌种资源的开发应用提供技术支持。To analyze the antioxidant properties of Lactobacillus and its relationship with katA gene.Six strains of Lactobacillus from naturally fermented meat products were selected as the research objects,and were evaluated by 1,1-diphenyl-2-picrylhydrazol(DPPH),hydroxyl(·OH),superoxide anion(O_(2)^(-)·)free radical scavenging rate,H_(2)O_(2) tolerance and katA gene expression.The results showed that the main active substances for scavenging·OH radicals were cell and intracellular substances,while scavenging DPPH and O_(2)^(-)·radicals were mainly metabolites of lactic acid bacteria.The scavenging ability of TR1-1-3 on the three kinds of free radicals was relatively strong,while the scavenging rate of·OH and O_(2)^(-)·of strain X31 was the lowest and the difference was significant(P<0.05)compared with TR1-1-3.In addition to strain X31,other experimental strains had a certain tolerance to H_(2)O_(2) in the range of 0-1.0 mmol/L H_(2)O_(2) concentration,and the tolerance gradually increased with the extension of the culture time within 12-48 hours.The katA gene of strain X31 with low free radical clearance and H_(2)O_(2) tolerance was negative by specific PCR,while all other strains were positive.This study found that lactic acid bacteria antioxidant capacity has strain specificity,through the apparent data of the scavenging rate of the free radicals of O_(2)^(-)·,·OH,DPPH and the tolerance to H_(2)O_(2) of the lactic acid bacteria,combined with the expression of katA gene,it can be used as the detection method for the determination of anti-oxidation lactic acid bacteria.The results can provide theoretical guidance and technical support for the development and application of high-quality strain resources.

关 键 词：乳酸菌 抗氧化特性 katA基因 自由基 

分 类 号：TS201.3[轻工技术与工程—食品科学]