高产生物膜乳酸菌的筛选、鉴定及胞外多糖分泌条件的研究  

Screening and Identification of High-producing Biofilm Lactic Acid Bacteria and Studies on the Conditions of Extracellular Polysaccharide Secretion

作　　者：钟华晨 张悦[1] 顾悦[1] 郑砚学 纪亚楠 王艳 贺银凤[1] Zhong Huachen;Zhang Yue;Gu Yue;Zheng Yanxue;Ji Yanan;Wang Yan;He Yinfeng(College of Food Science and Engineering,Inner Mongolia Agricultural University,Hohhot 010018;Institute of Resources,Environment and Detection Technology,Inner Mongolia Academy of Agriculture and Animal Husbandry Sciences,Hohhot 010031)

机构地区：[1]内蒙古农业大学食品科学与工程学院,呼和浩特010018 [2]内蒙古农牧业科学院资源环境与检测技术研究所,呼和浩特010031

出　　处：《中国食品学报》2021年第7期76-86,共11页Journal of Chinese Institute Of Food Science and Technology

基　　金：国家自然科学基金项目(31960467);内蒙古自治区自然科学基金项目(2019BS03002)。

摘　　要：生物膜是微生物在受到外界不良环境影响时产生的一种自我保护机制,多数研究集中在病原菌和有害菌。乳酸菌作为食品工业和人体肠道中重要的微生物,若以被膜态生长则可以更好地发挥其益生作用。胞外多糖是乳酸菌生长过程中分泌出的代谢产物,是生物膜的主要成分,乳酸菌胞外多糖分泌越多,预示着其产生物膜的能力越强。本研究对课题组前期分离出的41株乳酸菌,以高产生物膜为指标,筛选和鉴定出10株优良菌株。以1株乳酸片球菌RJ2-1-4为研究对象,探讨改变培养基成分对胞外多糖合成量的影响。乳酸片球菌RJ2-1-4产胞外多糖的最佳培养基组合为:葡萄糖20 g/L,大豆蛋白胨20 g/L,磷酸氢二钾8 g/L,此时EPS产量达到最大值为(427.83±18.40)mg/L。Biofilm is a self-protection mechanism produced by microorganisms when exposed to adverse external environment.Most researches focus on pathogenic bacteria and harmful bacteria.Lactic acid bacteria,as important microorganisms in the food industry and human intestinal tract,can better exert their probiotic effects if they are grown in a biofilm state.Extracellular polysaccharides(EPS)are metabolites secreted during the growth of lactic acid bacteria and are the main components of biofilms.The more secreted EPS from lactic acid bacteria,the stronger its ability to produce biofilms.In this study,41 strains of lactic acid bacteria were isolated in the early stage of the research group,and 10 excellent strains were screened and identified using the high-yield biofilm as an index.Taking a strain of Pediococcus lactis RJ2-1-4 as the research object,the effects of changing the composition of the medium on the amount of EPS were discussed.The optimal medium combination for EPS production by Pediococcus lactis RJ2-1-4 is:20 g/L glucose,20 g/L soy peptone,and 8 g/L dipotassium hydrogen phosphate.At this time,the EPS yield reaches the maximum value was(427.83±18.40)mg/L.

关 键 词：乳酸菌 高产生物膜 胞外多糖 培养基优化 

分 类 号：TS201.3[轻工技术与工程—食品科学]