塔什干地区发酵乳制品中酵母菌的分离鉴定  

Isolation and Identification of Yeast from Fermented Dairy Products in Toshkent

作　　者：如意 任冬艳 刘文俊[1] 孟和毕力格[1] RU Yi;REN Dongyan;LIU Wenjun;Bilige Menghe(Key Laboratory of Dairy Biotechnology and Engineering,Ministry of Education,Inner Mongolia Agricultural Univer-sity,Key Laboratory of Dairy Products Processing,Ministry of Agriculture and Rural Affairs,Inner Mongolia Key Labo-ratory of Dairy Biotechnology and Engineering,Hohhot 010018,China)

机构地区：[1]内蒙古农业大学乳品生物技术与工程教育部重点实验室,农业农村部奶制品加工重点实验室,内蒙古自治区乳品生物技术与工程重点实验室,呼和浩特010018

出　　处：《中国乳品工业》2021年第7期13-17,共5页China Dairy Industry

基　　金：内蒙古自治区科技计划项目(201802097)。

摘　　要：不同地区自然发酵乳由于地域和自然环境及制作方式的不同其微生物菌群组成均有一定的差异。本文对采集自乌兹别克斯坦塔什干地区的11份传统发酵乳中酵母菌进行了分离鉴定。采用纯培养方法分离出55株酵母菌,并利用26S rDNA D1/D2区序列分析和系统发育树分析对分离出的菌株进行分子生物学鉴定。从发酵乳样品中共鉴定出4个属4个种,分别为Kluyveromyces marxia⁃nus、Candida zeylanoides、Saccharomyces cerevisiae和Yarrowia lipolytica,其中Kluyveromyces marxianus为酸牛奶样品的主要分离菌株。塔什干地区发酵乳制品中的酵母菌具有丰富的生物多样性,为该地区发酵乳制品中酵母菌资源的研究和应用提供基础数据。The composition of microbial flora in natural fermented milk from different areas is different due to the differences of region,nat⁃ural environment and production methods.It was prefermented to isolate and identify yeast from 11 traditional fermented dairy samples col⁃lected from Toshkent in Uzbekistan in this study.A total of 55 strains of yeast were isolated by pure culture method,the species identifica⁃tion of yeast was carried out by molecular biology using 26S rDNA D1/D2 domain sequences analysis and phylogenetic tree analysis.Fif⁃ty-five yeast were isolated and identified into 4 genus and 4 species from fermented milk samples,including Kluyveromyces marxianus,Candida zeylanoides,Saccharomyces cerevisiae and Yarrowia lipolytica.The results showed that Kluyveromyces marxianus was the main isolated strain of yoghurt samples.The yeast in fermented dairy products in Toshkent is rich in biodiversity,which provides basic data for the research and application of yeast resources in fermented dairy products in this area。

关 键 词：发酵乳 酵母菌 酸牛奶 酸马奶 

分 类 号：Q93-331[生物学—微生物学]