香露兜粉的毒理学评价  

Toxicology Evaluation of Pandanus amaryllifolius Roxb Powder

作　　者：鱼欢[1] 张昂 马永忠[2] 宗迎[1] 冯丁山[2] 吉训志 秦晓威[1] YU Huan;ZHANG Ang;MA Yong-zhong;ZONG Ying;FENG Ding-Shan;JI Xun-zhi;QIN Xiao-wei(Spice and Beverage Research Institute,Chinese Academy of Tropical Agricultural Sciences,Hainan Provincial Key Laboratory of Genetic Improvement and Quality Regulation for Tropical Spice and Beverage Crops,Key Laboratory of Genetic Resource Utilization of Spice and Beverage Crops,Ministry of Agriculture and Rural Affairs,Wanning 571533,China;Hainan Provincial Centre for Disease Control and Prevention,Haikou 570203,China)

机构地区：[1]中国热带农业科学院香料饮料研究所,海南省热带香辛饮料作物遗传改良与品质调控重点实验室,农业农村部香辛饮料作物遗传资源利用重点实验室,海南万宁571533 [2]海南省疾病预防控制中心,海南海口570203

出　　处：《现代食品科技》2021年第9期263-270,共8页Modern Food Science and Technology

基　　金：中国热带农业科学院基本科研业务费(1630142020014,1630142018014)。

摘　　要：该文主要研究了食用香露兜粉对大鼠的亚慢性毒性,为相关食品的开发提供毒理学安全性依据。研究依照GB 15193.13-2015《食品安全国家标准90天经口毒性试验》的方法,将大鼠随机分为对照组以及香露兜粉样品低剂量组(2.00 g/kg BW)、中剂量组(4.00 g/kg BW)、高剂量组(8.00 g/kg BW),每组20只,雌雄各半。通过一般临床观察、体重和摄食、眼部检查、血液学及血生化学检查、尿液检查、大体解剖与组织病理学检查等评估香露兜粉亚慢性毒性。试验期间大鼠生长发育良好,雄鼠与雌鼠分别增重438 g和274 g,食物利用率分别为17.4%和13.2%,并且香露兜粉各剂量组体重、增重、食物利用率与处理对照相比无显著差异(p>0.05)。此外,不同香露兜粉剂量组雄鼠与雌鼠的各项血常规指标、血生化指标、凝血功能指标、脏器重量及脏器/体重比值与对照处理相比均无显著性差异(p>0.05)。尿常规指标、检眼镜检查及大体解剖和组织病理检查未见明显与样品有关的异常改变。本次香露兜粉90 d经口毒性试验无可见有害作用水平(NOAEL)为雄鼠8.89 g/kg BW,雌鼠9.76 g/kg BW。综上所述,在本试验条件下,香露兜粉样品对大鼠不具有亚慢性毒性作用。The subchronic toxicity of Pandanus amaryllifolius Roxb.powder on rats was evaluated,which was expected to provide toxicological safety basis for the development of food additions.The rats were randomly divided into the control group and Pandanus amaryllifolius Roxb.powder samples of low dose group(2.00 g/kg BW),middle dose group(4.00 g/kg BW),high dose group(8.00 g/kg BW),using the oral toxicity test method according to GB 15193.13-2015 in this study.There were 20 rats in each group,half male and half female.Oral toxicity of Pandanus amaryllifolius Roxb.powder was assessed by general clinical observation,weight and feeding,eye examination,hematology and biochemistry,urine examination,gross anatomy and histopathology.During the experiment,the rats’growth was good.There were no significant differences in body weight,weight gain,food utilization rate,blood routine indexes,blood biochemical indexes,blood coagulation function indexes,organ weight and organ/body weight ratio among each Pandanus amaryllifolius Roxb.powder dose group and the control group(p>0.05).No abnormal changes were observed in routine urine,ophthalmoscopics,gross anatomy and histopathology.The no observed adverse effect levels(NOAEL)of Pandanus amaryllifolius Roxb.powder dose were 8.89 g/kg BW in male and 9.76 g/kg BW in female.There was no subchronic toxicity of Pandanus amaryllifolius Roxb.powdersamplesto rats in this study.

关 键 词：香露兜粉 大鼠 亚慢性毒性 食用安全性 

分 类 号：TS201.4[轻工技术与工程—食品科学]